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Single-strand RPA for rapid and sensitive detection of SARS-CoV-2 RNA
We report the single-strand Recombinase Polymerase Amplification (ssRPA) method, which merges the fast, isothermal amplification of RPA with subsequent rapid conversion of the double-strand DNA amplicon to single strands, and hence enables facile hybridization-based, high-specificity readout. We dem...
Autores principales: | Kim, Youngeun, Yaseen, Adam B., Kishi, Jocelyn Y., Hong, Fan, Saka, Sinem K., Sheng, Kuanwei, Gopalkrishnan, Nikhil, Schaus, Thomas E., Yin, Peng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7444299/ https://www.ncbi.nlm.nih.gov/pubmed/32839783 http://dx.doi.org/10.1101/2020.08.17.20177006 |
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