Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence

CRISPR/Cas-mediated genome editing is a powerful tool for generating genetically mutated cells and organisms. Linearisation of donor cassettes with this system has been shown to facilitate both transgene donor insertion and targeted knock-in. Here, we developed a donor plasmid that we name pCriMGET...

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Detalles Bibliográficos
Autores principales: Ishibashi, Riki, Abe, Kota, Ido, Nanami, Kitano, Satsuki, Miyachi, Hitoshi, Toyoshima, Fumiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445171/
https://www.ncbi.nlm.nih.gov/pubmed/32839482
http://dx.doi.org/10.1038/s41598-020-70804-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445171/
https://www.ncbi.nlm.nih.gov/pubmed/32839482
http://dx.doi.org/10.1038/s41598-020-70804-6

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