Optimization of the experimental parameters of the ligase cycling reaction

The ligase cycling reaction (LCR) is a scarless and efficient method to assemble plasmids from fragments of DNA. This assembly method is based on the hybridization of DNA fragments with complementary oligonucleotides, so-called bridging oligos (BOs), and an experimental procedure of thermal denatura...

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Detalles Bibliográficos
Autores principales: Schlichting, Niels, Reinhardt, Felix, Jager, Sven, Schmidt, Michael, Kabisch, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445781/
https://www.ncbi.nlm.nih.gov/pubmed/32995543
http://dx.doi.org/10.1093/synbio/ysz020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445781/
https://www.ncbi.nlm.nih.gov/pubmed/32995543
http://dx.doi.org/10.1093/synbio/ysz020

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