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Optimization of the experimental parameters of the ligase cycling reaction
The ligase cycling reaction (LCR) is a scarless and efficient method to assemble plasmids from fragments of DNA. This assembly method is based on the hybridization of DNA fragments with complementary oligonucleotides, so-called bridging oligos (BOs), and an experimental procedure of thermal denatura...
Autores principales: | Schlichting, Niels, Reinhardt, Felix, Jager, Sven, Schmidt, Michael, Kabisch, Johannes |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445781/ https://www.ncbi.nlm.nih.gov/pubmed/32995543 http://dx.doi.org/10.1093/synbio/ysz020 |
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