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A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E

BACKGROUND: Currently, one of the most reliable methods for viral infection detection are polymerase chain reaction (PCR) based assays. This process is time and resource heavy, requiring multiple steps of lysis, extraction, purification, and amplification procedures. Herein, we have developed a meth...

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Detalles Bibliográficos
Autores principales: Morehouse, Zachary P., Proctor, Caleb M., Ryan, Gabriella L., Nash, Rodney J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445803/
https://www.ncbi.nlm.nih.gov/pubmed/32843049
http://dx.doi.org/10.1186/s12985-020-01405-y