Cargando…
A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E
BACKGROUND: Currently, one of the most reliable methods for viral infection detection are polymerase chain reaction (PCR) based assays. This process is time and resource heavy, requiring multiple steps of lysis, extraction, purification, and amplification procedures. Herein, we have developed a meth...
Autores principales: | Morehouse, Zachary P., Proctor, Caleb M., Ryan, Gabriella L., Nash, Rodney J. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7445803/ https://www.ncbi.nlm.nih.gov/pubmed/32843049 http://dx.doi.org/10.1186/s12985-020-01405-y |
Ejemplares similares
-
693. Shaking Things Up: Direct-to-PCR Viral Detection off Swabs Using Shaker-Mill Homogenization
por: Morehouse, Zachary P, et al.
Publicado: (2020) -
Validation of a direct-to-PCR COVID-19 detection protocol utilizing mechanical homogenization: A model for reducing resources needed for accurate testing
por: Morehouse, Zachary P., et al.
Publicado: (2021) -
Oligomerization of the carboxyl terminal domain of the human coronavirus 229E nucleocapsid protein
por: Lo, Yu-Sheng, et al.
Publicado: (2013) -
PCR sequencing of the spike genes of geographically and chronologically distinct human coronaviruses 229E
por: Hays, J.P., et al.
Publicado: (1998) -
Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
por: Lee, Hyun-Kyoung, et al.
Publicado: (2010)