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Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis

Chinese hamster ovary cells have been the workhorse for the production of recombinant proteins in mammalian cells. Since biochemical, cellular and omics studies are usually affected by the lack of suitable fractionation procedures to isolate compartments from these cells, differential and isopycnic...

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Detalles Bibliográficos
Autores principales: Pérez-Rodriguez, Saumel, de Jesús Ramírez-Lira, María, Wulff, Tune, Voldbor, Bjørn Gunnar, Ramírez, Octavio T., Trujillo-Roldán, Mauricio A., Valdez-Cruz, Norma A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447005/
https://www.ncbi.nlm.nih.gov/pubmed/32841274
http://dx.doi.org/10.1371/journal.pone.0237930