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Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis
Chinese hamster ovary cells have been the workhorse for the production of recombinant proteins in mammalian cells. Since biochemical, cellular and omics studies are usually affected by the lack of suitable fractionation procedures to isolate compartments from these cells, differential and isopycnic...
Autores principales: | Pérez-Rodriguez, Saumel, de Jesús Ramírez-Lira, María, Wulff, Tune, Voldbor, Bjørn Gunnar, Ramírez, Octavio T., Trujillo-Roldán, Mauricio A., Valdez-Cruz, Norma A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447005/ https://www.ncbi.nlm.nih.gov/pubmed/32841274 http://dx.doi.org/10.1371/journal.pone.0237930 |
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