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Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents
BACKGROUND: Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the applicatio...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7449025/ https://www.ncbi.nlm.nih.gov/pubmed/32843026 http://dx.doi.org/10.1186/s12896-020-00636-9 |