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Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans

Although traditional egg-based inactivated influenza vaccines can protect against infection, there have been significant efforts to develop improved formats to overcome disadvantages of this platform. Here, we have assessed human CD4 T cell responses to a traditional egg-based influenza vaccine with...

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Autores principales: Richards, K. A., Moritzky, S., Shannon, I., Fitzgerald, T., Yang, H., Branche, A., Topham, D. J., Treanor, J. J., Nayak, J., Sant, Andrea J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450042/
https://www.ncbi.nlm.nih.gov/pubmed/32884842
http://dx.doi.org/10.1038/s41541-020-00227-x
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author Richards, K. A.
Moritzky, S.
Shannon, I.
Fitzgerald, T.
Yang, H.
Branche, A.
Topham, D. J.
Treanor, J. J.
Nayak, J.
Sant, Andrea J.
author_facet Richards, K. A.
Moritzky, S.
Shannon, I.
Fitzgerald, T.
Yang, H.
Branche, A.
Topham, D. J.
Treanor, J. J.
Nayak, J.
Sant, Andrea J.
author_sort Richards, K. A.
collection PubMed
description Although traditional egg-based inactivated influenza vaccines can protect against infection, there have been significant efforts to develop improved formats to overcome disadvantages of this platform. Here, we have assessed human CD4 T cell responses to a traditional egg-based influenza vaccine with recently available cell-derived vaccines and recombinant baculovirus-derived vaccines. Adults were administered either egg-derived Fluzone(®), mammalian cell-derived Flucelvax(®) or recombinant HA (Flublok(®)). CD4 T cell responses to each HA protein were assessed by cytokine EliSpot and intracellular staining assays. The specificity and magnitude of antibody responses were quantified by ELISA and HAI assays. By all criteria, Flublok vaccine exhibited superior performance in eliciting both CD4 T cell responses and HA-specific antibody responses, whether measured by mean response magnitude or percent of responders. Although the mechanism(s) underlying this advantage is not yet clear, it is likely that both qualitative and quantitative features of the vaccines impact the response.
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spelling pubmed-74500422020-09-02 Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans Richards, K. A. Moritzky, S. Shannon, I. Fitzgerald, T. Yang, H. Branche, A. Topham, D. J. Treanor, J. J. Nayak, J. Sant, Andrea J. NPJ Vaccines Article Although traditional egg-based inactivated influenza vaccines can protect against infection, there have been significant efforts to develop improved formats to overcome disadvantages of this platform. Here, we have assessed human CD4 T cell responses to a traditional egg-based influenza vaccine with recently available cell-derived vaccines and recombinant baculovirus-derived vaccines. Adults were administered either egg-derived Fluzone(®), mammalian cell-derived Flucelvax(®) or recombinant HA (Flublok(®)). CD4 T cell responses to each HA protein were assessed by cytokine EliSpot and intracellular staining assays. The specificity and magnitude of antibody responses were quantified by ELISA and HAI assays. By all criteria, Flublok vaccine exhibited superior performance in eliciting both CD4 T cell responses and HA-specific antibody responses, whether measured by mean response magnitude or percent of responders. Although the mechanism(s) underlying this advantage is not yet clear, it is likely that both qualitative and quantitative features of the vaccines impact the response. Nature Publishing Group UK 2020-08-26 /pmc/articles/PMC7450042/ /pubmed/32884842 http://dx.doi.org/10.1038/s41541-020-00227-x Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Richards, K. A.
Moritzky, S.
Shannon, I.
Fitzgerald, T.
Yang, H.
Branche, A.
Topham, D. J.
Treanor, J. J.
Nayak, J.
Sant, Andrea J.
Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans
title Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans
title_full Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans
title_fullStr Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans
title_full_unstemmed Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans
title_short Recombinant HA-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific CD4 T cells and CD4 T cell-dependent antibody responses in humans
title_sort recombinant ha-based vaccine outperforms split and subunit vaccines in elicitation of influenza-specific cd4 t cells and cd4 t cell-dependent antibody responses in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7450042/
https://www.ncbi.nlm.nih.gov/pubmed/32884842
http://dx.doi.org/10.1038/s41541-020-00227-x
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