Cargando…

Single-Stranded Phosphorothioated Regions Enhance Cellular Uptake of Cholesterol-Conjugated siRNA but Not Silencing Efficacy

Small interfering RNAs (siRNAs) have potential to silence virtually any disease-causing gene but require chemical modifications for delivery to the tissue and cell of interest. Previously, we demonstrated that asymmetric, phosphorothioate (PS)-modified, chemically stabilized, cholesterol-conjugated...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ly, Socheata, Echeverria, Dimas, Sousa, Jacquelyn, Khvorova, Anastasia
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452107/ https://www.ncbi.nlm.nih.gov/pubmed/32818923 http://dx.doi.org/10.1016/j.omtn.2020.07.029

Ejemplares similares

The chemical structure and phosphorothioate content of hydrophobically modified siRNAs impact extrahepatic distribution and efficacy
por: Biscans, Annabelle, et al.
Publicado: (2020)

Cell Type Impacts Accessibility of mRNA to Silencing by RNA Interference
por: Ferguson, Chantal M., et al.
Publicado: (2020)

2′-O-Methyl at 20-mer Guide Strand 3′ Termini May Negatively Affect Target Silencing Activity of Fully Chemically Modified siRNA
por: Davis, Sarah M., et al.
Publicado: (2020)

Hydrophobicity drives the systemic distribution of lipid-conjugated siRNAs via lipid transport pathways
por: Osborn, Maire F, et al.
Publicado: (2019)

Diverse lipid conjugates for functional extra-hepatic siRNA delivery in vivo
por: Biscans, Annabelle, et al.
Publicado: (2019)

Visualization of self-delivering hydrophobically modified siRNA cellular internalization
por: Ly, Socheata, et al.
Publicado: (2017)

Chemical optimization of siRNA for safe and efficient silencing of placental sFLT1
por: Davis, Sarah M., et al.
Publicado: (2022)

5΄-Vinylphosphonate improves tissue accumulation and efficacy of conjugated siRNAs in vivo
por: Haraszti, Reka A., et al.
Publicado: (2017)

Functional features defining the efficacy of cholesterol-conjugated, self-deliverable, chemically modified siRNAs
por: Shmushkovich, Taisia, et al.
Publicado: (2018)

Docosahexaenoic Acid Conjugation Enhances Distribution and Safety of siRNA upon Local Administration in Mouse Brain
por: Nikan, Mehran, et al.
Publicado: (2016)

Silencing Myostatin Using Cholesterol-conjugated siRNAs Induces Muscle Growth
por: Khan, Tayeba, et al.
Publicado: (2016)

Di-valent siRNA-mediated silencing of MSH3 blocks somatic repeat expansion in mouse models of Huntington’s disease
por: O’Reilly, Daniel, et al.
Publicado: (2023)

Chemical engineering of therapeutic siRNAs for allele-specific gene silencing in Huntington’s disease models
por: Conroy, Faith, et al.
Publicado: (2022)

Ligand Conjugated Multimeric siRNAs Enable Enhanced Uptake and Multiplexed Gene Silencing
por: Brown, Jonathan M., et al.
Publicado: (2019)

High potency silencing by single-stranded boranophosphate siRNA
por: Hall, Allison H. S., et al.
Publicado: (2006)

Comparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo
por: Hassler, Matthew R, et al.
Publicado: (2018)

Docosanoic acid conjugation to siRNA enables functional and safe delivery to skeletal and cardiac muscles
por: Biscans, Annabelle, et al.
Publicado: (2021)

Guanabenz (Wytensin™) selectively enhances uptake and efficacy of hydrophobically modified siRNAs
por: Osborn, Maire F., et al.
Publicado: (2015)

The siRNA sequence and guide strand overhangs are determinants of in vivo duration of silencing
por: Strapps, Walter R., et al.
Publicado: (2010)

Stereochemical bias introduced during RNA synthesis modulates the activity of phosphorothioate siRNAs
por: Jahns, Hartmut, et al.
Publicado: (2015)

An artificial cationic oligosaccharide combined with phosphorothioate linkages strongly improves siRNA stability
por: Irie, Atsushi, et al.
Publicado: (2020)

Disulfide-Based Poly(amido amine)s for siRNA Delivery: Effects of Structure on siRNA Complexation, Cellular Uptake, Gene Silencing and Toxicity
por: Vader, Pieter, et al.
Publicado: (2010)

In Vitro Cellular Uptake and Transfection of Oligoarginine-Conjugated Glycol Chitosan/siRNA Nanoparticles
por: Jeong, Eun-Ju, et al.
Publicado: (2021)

Effective carrier-free gene-silencing activity of cholesterol-modified siRNAs
por: Salim, Lidya, et al.
Publicado: (2018)

Defined Folate-PEG-siRNA Conjugates for Receptor-specific Gene Silencing
por: Dohmen, Christian, et al.
Publicado: (2012)

Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group
por: Petrova, Natalya S., et al.
Publicado: (2012)

Hydrophobically Modified siRNAs Silence Huntingtin mRNA in Primary Neurons and Mouse Brain
por: Alterman, Julia F, et al.
Publicado: (2015)

Both Strands of siRNA Have Potential to Guide Posttranscriptional Gene Silencing in Mammalian Cells
por: Wei, Jun-Xia, et al.
Publicado: (2009)

Chemically defined polyethylene glycol siRNA conjugates with enhanced gene silencing effect
por: Gaziova, Zuzana, et al.
Publicado: (2014)

Feasibility of cRGD conjugation at 5′-antisense strand of siRNA by phosphodiester linkage extension
por: Zhou, Xinyang, et al.
Publicado: (2021)

PK-modifying anchors significantly alter clearance kinetics, tissue distribution, and efficacy of therapeutics siRNAs
por: Godinho, Bruno M.D.C., et al.
Publicado: (2022)

Mutant huntingtin messenger RNA forms neuronal nuclear clusters in rodent and human brains
por: Ly, Socheata, et al.
Publicado: (2022)

Thermal Stability of siRNA Modulates Aptamer- conjugated siRNA Inhibition
por: Berezhnoy, Alexey, et al.
Publicado: (2012)

Bridging siRNA strands for better function
por: Rossi, John J.
Publicado: (2023)

Asymmetric trichotomous partitioning overcomes dataset limitations in building machine learning models for predicting siRNA efficacy
por: Monopoli, Kathryn R., et al.
Publicado: (2023)

Structurally constrained phosphonate internucleotide linkage impacts oligonucleotide-enzyme interaction, and modulates siRNA activity and allele specificity
por: Yamada, Ken, et al.
Publicado: (2021)

Impact of Serum Proteins on the Uptake and RNAi Activity of GalNAc-Conjugated siRNAs
por: Agarwal, Saket, et al.
Publicado: (2021)

Therapeutic siRNA silencing in inflammatory monocytes
por: Leuschner, Florian, et al.
Publicado: (2011)

Quantitative Silencing of EGFP Reporter Gene by Self-Assembled siRNA Lipoplexes of LinOS and Cholesterol
por: Metwally, Abdelkader A., et al.
Publicado: (2012)

Divalent siRNAs are bioavailable in the lung and efficiently block SARS-CoV-2 infection
por: Hariharan, Vignesh N., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_0lm8309e37obc85ljjcfoda1k3