Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice

Dengue virus (DENV) infection is a major global public health concern, and there is no effective vaccine for it. In this study, we describe the design and characterization of three nucleotide-modified mRNA vaccines (prME-mRNA, E80-mRNA, and NS1-mRNA) for DENV-2. Our results showed that vaccination w...

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Detalles Bibliográficos
Autores principales: Zhang, Mengling, Sun, Jin, Li, Min, Jin, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452130/
https://www.ncbi.nlm.nih.gov/pubmed/32913878
http://dx.doi.org/10.1016/j.omtm.2020.07.013
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author Zhang, Mengling
Sun, Jin
Li, Min
Jin, Xia
author_facet Zhang, Mengling
Sun, Jin
Li, Min
Jin, Xia
author_sort Zhang, Mengling
collection PubMed
description Dengue virus (DENV) infection is a major global public health concern, and there is no effective vaccine for it. In this study, we describe the design and characterization of three nucleotide-modified mRNA vaccines (prME-mRNA, E80-mRNA, and NS1-mRNA) for DENV-2. Our results showed that vaccination with E80-mRNA alone or a combination of E80-mRNA and NS1-mRNA can induce high levels of neutralizing antibodies and antigen-specific T cell responses; furthermore, these vaccines confer complete protection against DENV-2 challenge in immunocompetent mice. These data provide foundations for further development of a tetravalent DENV vaccine based on nucleotide-modified mRNA.
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spelling pubmed-74521302020-09-09 Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice Zhang, Mengling Sun, Jin Li, Min Jin, Xia Mol Ther Methods Clin Dev Article Dengue virus (DENV) infection is a major global public health concern, and there is no effective vaccine for it. In this study, we describe the design and characterization of three nucleotide-modified mRNA vaccines (prME-mRNA, E80-mRNA, and NS1-mRNA) for DENV-2. Our results showed that vaccination with E80-mRNA alone or a combination of E80-mRNA and NS1-mRNA can induce high levels of neutralizing antibodies and antigen-specific T cell responses; furthermore, these vaccines confer complete protection against DENV-2 challenge in immunocompetent mice. These data provide foundations for further development of a tetravalent DENV vaccine based on nucleotide-modified mRNA. American Society of Gene & Cell Therapy 2020-07-21 /pmc/articles/PMC7452130/ /pubmed/32913878 http://dx.doi.org/10.1016/j.omtm.2020.07.013 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Zhang, Mengling
Sun, Jin
Li, Min
Jin, Xia
Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice
title Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice
title_full Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice
title_fullStr Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice
title_full_unstemmed Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice
title_short Modified mRNA-LNP Vaccines Confer Protection against Experimental DENV-2 Infection in Mice
title_sort modified mrna-lnp vaccines confer protection against experimental denv-2 infection in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7452130/
https://www.ncbi.nlm.nih.gov/pubmed/32913878
http://dx.doi.org/10.1016/j.omtm.2020.07.013
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