Cargando…

Effect of pre-freezing and saccharide types in freeze-drying of siRNA lipoplexes on gene-silencing effects in the cells by reverse transfection

Our previous study reported that reverse (Rev)-transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) freeze-dried in trehalose or sucrose solution resulted in high gene-silencing activity in cells. The current study investigated whether pre-freezing or saccha...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tang, Min, Hu, Subin, Hattori, Yoshiyuki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	D.A. Spandidos 2020
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7453497/ https://www.ncbi.nlm.nih.gov/pubmed/32945442 http://dx.doi.org/10.3892/mmr.2020.11419

Ejemplares similares

Fate of Tableted Freeze-Dried siRNA Lipoplexes in Gastrointestinal Environment
por: Rehman, Asad Ur, et al.
Publicado: (2021)

Effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene-silencing effects and siRNA biodistribution in mice
por: Hattori, Yoshiyuki, et al.
Publicado: (2020)

Protective Effect of Saccharides on Freeze-Dried Liposomes Encapsulating Drugs
por: Guimarães, Diana, et al.
Publicado: (2019)

Effects of sterol derivatives in cationic liposomes on biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes
por: Hattori, Yoshiyuki, et al.
Publicado: (2021)

Freeze Drying of Polymer Nanoparticles and Liposomes Exploiting Different Saccharide-Based Approaches
por: Andreana, Ilaria, et al.
Publicado: (2023)

Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs
por: Hattori, Yoshiyuki, et al.
Publicado: (2023)

In vivo siRNA delivery system for targeting to the liver by poly-l-glutamic acid-coated lipoplex()
por: Hattori, Yoshiyuki, et al.
Publicado: (2014)

Quantitative Silencing of EGFP Reporter Gene by Self-Assembled siRNA Lipoplexes of LinOS and Cholesterol
por: Metwally, Abdelkader A., et al.
Publicado: (2012)

The Effect of Pre-Treatment (Blanching, Ultrasound and Freezing) on Quality of Freeze-Dried Red Beets
por: Ciurzyńska, Agnieszka, et al.
Publicado: (2021)

The Effect of Freezing and Freeze-drying on the Transplantation of Sarcoma 37
por: Warner, P. T. J. C. P., et al.
Publicado: (1950)

Neuroprotective Effect of siRNA Entrapped in Hyaluronic Acid-Coated Lipoplexes by Intravitreal Administration
por: Ribeiro, Marcela Coelho Silva, et al.
Publicado: (2021)

Combinations of Freeze-Dried Amorphous Vardenafil Hydrochloride with Saccharides as a Way to Enhance Dissolution Rate and Permeability
por: Wiergowska, Gabriela, et al.
Publicado: (2021)

Development of siRNA lipoplexes for intracellular delivery in lung cancer cells
por: Khatri, N., et al.
Publicado: (2012)

Effective Delivery of PEGylated siRNA-Containing Lipoplexes to Extraperitoneal Tumours following Intraperitoneal Administration
por: Singhania, Akul, et al.
Publicado: (2011)

Optimal combination of cationic lipid and phospholipid in cationic liposomes for gene knockdown in breast cancer cells and mouse lung using siRNA lipoplexes
por: Hattori, Yoshiyuki, et al.
Publicado: (2022)

Self-Assembled Lipoplexes of Short Interfering RNA (siRNA) Using Spermine-Based Fatty Acid Amide Guanidines: Effect on Gene Silencing Efficiency
por: Metwally, Abdelkader A., et al.
Publicado: (2011)

Exploring the Correlation Between Lipid Packaging in Lipoplexes and Their Transfection Efficacy
por: Moghaddam, Behfar, et al.
Publicado: (2011)

Effect of Cationic Lipid Type in Folate-PEG-Modified Cationic Liposomes on Folate Receptor-Mediated siRNA Transfection in Tumor Cells
por: Hattori, Yoshiyuki, et al.
Publicado: (2019)

Effect of Pulsed Electric Field Pre-Treatment and the Freezing Methods on the Kinetics of the Freeze-Drying Process of Apple and Its Selected Physical Properties
por: Nowak, Dorota, et al.
Publicado: (2022)

Effect of Encapsulation Techniques on Aroma Retention of Pistacia terebinthus L. Fruit Oil: Spray Drying, Spray Freeze Drying, and Freeze Drying
por: Yaman, Delal Meryem, et al.
Publicado: (2023)

Cryopreservation and freeze-drying protocols /
Publicado: (2015)

Cryopreservation and freeze-drying protocols /
Publicado: (1995)

Freeze-Drying Technology in Foods
por: Prosapio, Valentina, et al.
Publicado: (2020)

Targeted Delivery of siRNA Lipoplexes to Cancer Cells Using Macrophage Transient Horizontal Gene Transfer
por: Wayne, Elizabeth C., et al.
Publicado: (2019)

Combined Inhibition of FOSL-1 and YAP Using siRNA-Lipoplexes Reduces the Growth of Pancreatic Tumor
por: Diego-González, Lara, et al.
Publicado: (2022)

To freeze or not to freeze
por: Bangasser, Debra
Publicado: (2015)

Development and Application of a Mechanistic Cooling and Freezing Model of the Spin Freezing Step within the Framework of Continuous Freeze-Drying
por: Nuytten, Gust, et al.
Publicado: (2021)

Long-Circulating and Fusogenic Liposomes Loaded with Paclitaxel and Doxorubicin: Effect of Excipient, Freezing, and Freeze-Drying on Quality Attributes
por: Roque, Marjorie, et al.
Publicado: (2022)

Stains from Freeze-Dried Drops
por: Jambon-Puillet, Etienne
Publicado: (2019)

Freeze-Drying of Plant-Based Foods
por: Bhatta, Sagar, et al.
Publicado: (2020)

Freeze-drying for the preservation of immunoengineering products
por: Kommineni, Nagavendra, et al.
Publicado: (2022)

The Effect of Composition, Pre-Treatment on the Mechanical and Acoustic Properties of Apple Gels and Freeze-Dried Materials
por: Jakubczyk, Ewa, et al.
Publicado: (2022)

Effect of vacuum freeze drying and hot air drying on dried mulberry fruit quality
por: Wang, Li, et al.
Publicado: (2023)

The Impact of Freeze-Drying Conditions on the Physico-Chemical Properties and Bioactive Compounds of a Freeze-Dried Orange Puree
por: Silva-Espinoza, Marilú A., et al.
Publicado: (2019)

Physico-Chemical Characteristics of Lipoplexes Influence Cell Uptake Mechanisms and Transfection Efficacy
por: Resina, Sarah, et al.
Publicado: (2009)

Multi-Level Kinetic Model of mRNA Delivery via Transfection of Lipoplexes
por: Ligon, Thomas S., et al.
Publicado: (2014)

Increasing Transfection Efficiency of Lipoplexes by Modulating Complexation Solution for Transient Gene Expression
por: Kim, Jaemun, et al.
Publicado: (2021)

Is pre-freeze sperm preparation more advantageous than post-freeze?
por: Androni, Dimitra Apostolia, et al.
Publicado: (2021)

Preparation of Chitosan Nanocompositeswith a Macroporous Structure by Unidirectional Freezing and Subsequent Freeze-Drying
por: Aranaz, Inmaculada, et al.
Publicado: (2014)

Effects of gliadin and glutenin on the hygroscopicity of freeze-dried apple powders
por: Yang, Xiaotong, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_9c9eici42edlh4sr4hk3ooe6oj