Cargando…
Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria
Severely elevated plasma homocysteine and methionine lead to thromboembolic events and strokes in homocystinuric (HCU) patients. Mouse models of HCU failed to exhibit prothrombotic phenotype, presumably due to lack of hypermethioninemia. We evaluated the impact of hypermethioninemia together with hy...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459533/ https://www.ncbi.nlm.nih.gov/pubmed/32722248 http://dx.doi.org/10.3390/biomedicines8080244 |
_version_ | 1783576396703989760 |
---|---|
author | Park, Insun Johnson, Linda K. Cox, Allaura Branchford, Brian R. Paola, Jorge Di Bublil, Erez M. Majtan, Tomas |
author_facet | Park, Insun Johnson, Linda K. Cox, Allaura Branchford, Brian R. Paola, Jorge Di Bublil, Erez M. Majtan, Tomas |
author_sort | Park, Insun |
collection | PubMed |
description | Severely elevated plasma homocysteine and methionine lead to thromboembolic events and strokes in homocystinuric (HCU) patients. Mouse models of HCU failed to exhibit prothrombotic phenotype, presumably due to lack of hypermethioninemia. We evaluated the impact of hypermethioninemia together with hyperhomocysteinemia on murine HCU phenotype and compared the efficacy of the current and novel therapies for HCU. High methionine intake decreased survival of I278T mice, which died from intestinal bleeding with hepatic and pancreatic failure. I278T mice on normal or increased methionine intake developed endothelial dysfunction, but paradoxically demonstrated delayed occlusion in an induced arterial thrombosis model. RNA-seq analysis suggested that expression of coagulation factor XI (FXI) is downregulated in livers of I278T mice. Indeed, plasma concentrations of FXI were decreased in I278T mice on normal diet and further reduced by increased methionine intake. Dietary methionine restriction normalized the observed phenotype. Similarly, treatment with OT-58, a novel enzyme therapy for HCU, corrected the phenotype in I278T mice regardless of their dietary methionine intake. Hypermethioninemia does not contribute to prothrombotic phenotype in murine HCU. Downregulation of FXI may contribute to the lack of prothrombotic tendency in I278T mice. Methionine restriction or treatment with OT-58 corrects vascular disease in the I278T mouse model of HCU. |
format | Online Article Text |
id | pubmed-7459533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74595332020-09-02 Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria Park, Insun Johnson, Linda K. Cox, Allaura Branchford, Brian R. Paola, Jorge Di Bublil, Erez M. Majtan, Tomas Biomedicines Article Severely elevated plasma homocysteine and methionine lead to thromboembolic events and strokes in homocystinuric (HCU) patients. Mouse models of HCU failed to exhibit prothrombotic phenotype, presumably due to lack of hypermethioninemia. We evaluated the impact of hypermethioninemia together with hyperhomocysteinemia on murine HCU phenotype and compared the efficacy of the current and novel therapies for HCU. High methionine intake decreased survival of I278T mice, which died from intestinal bleeding with hepatic and pancreatic failure. I278T mice on normal or increased methionine intake developed endothelial dysfunction, but paradoxically demonstrated delayed occlusion in an induced arterial thrombosis model. RNA-seq analysis suggested that expression of coagulation factor XI (FXI) is downregulated in livers of I278T mice. Indeed, plasma concentrations of FXI were decreased in I278T mice on normal diet and further reduced by increased methionine intake. Dietary methionine restriction normalized the observed phenotype. Similarly, treatment with OT-58, a novel enzyme therapy for HCU, corrected the phenotype in I278T mice regardless of their dietary methionine intake. Hypermethioninemia does not contribute to prothrombotic phenotype in murine HCU. Downregulation of FXI may contribute to the lack of prothrombotic tendency in I278T mice. Methionine restriction or treatment with OT-58 corrects vascular disease in the I278T mouse model of HCU. MDPI 2020-07-24 /pmc/articles/PMC7459533/ /pubmed/32722248 http://dx.doi.org/10.3390/biomedicines8080244 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Insun Johnson, Linda K. Cox, Allaura Branchford, Brian R. Paola, Jorge Di Bublil, Erez M. Majtan, Tomas Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria |
title | Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria |
title_full | Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria |
title_fullStr | Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria |
title_full_unstemmed | Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria |
title_short | Hypermethioninemia Leads to Fatal Bleeding and Increased Mortality in a Transgenic I278T Mouse Model of Homocystinuria |
title_sort | hypermethioninemia leads to fatal bleeding and increased mortality in a transgenic i278t mouse model of homocystinuria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459533/ https://www.ncbi.nlm.nih.gov/pubmed/32722248 http://dx.doi.org/10.3390/biomedicines8080244 |
work_keys_str_mv | AT parkinsun hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria AT johnsonlindak hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria AT coxallaura hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria AT branchfordbrianr hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria AT paolajorgedi hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria AT bublilerezm hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria AT majtantomas hypermethioninemialeadstofatalbleedingandincreasedmortalityinatransgenici278tmousemodelofhomocystinuria |