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Mass-Spectrometry Based Proteome Comparison of Extracellular Vesicle Isolation Methods: Comparison of ME-kit, Size-Exclusion Chromatography, and High-Speed Centrifugation

Extracellular vesicles (EVs) are small membrane-enclosed particles released by cells under various conditions specific to cells’ biological states. Hence, mass-spectrometry (MS) based proteome analysis of EVs in plasma has gained much attention as a method to discover novel protein biomarkers. MS an...

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Detalles Bibliográficos
Autores principales: Askeland, Anders, Borup, Anne, Østergaard, Ole, Olsen, Jesper V., Lund, Sigrid M., Christiansen, Gunna, Kristensen, Søren R., Heegaard, Niels H. H., Pedersen, Shona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459681/
https://www.ncbi.nlm.nih.gov/pubmed/32722497
http://dx.doi.org/10.3390/biomedicines8080246