Effects of Flaxseed Oil and Vitamin E Supplementation on Digestibility and Milk Fatty Composition and Antioxidant Capacity in Water Buffaloes

SIMPLE SUMMARY: Flaxseed oil is rich in n-3 fatty acids, while vitamin E is a potent antioxidant. Both have been tested in dairy cows’ diets to increase n-3 concentration and antioxidant capacity in the milk. However, there is no published research testing flaxseed oil and vitamin E supplementation simultaneously in lactating dairy buffaloes, which can have a different response compared to dairy cows. Increasing milk unsaturated fatty acids while not increasing lipid oxidation is a challenge; however, in this experiment we demonstrated that it is possible to achieve these in buffalo milk by supplementing the diet with flaxseed oil and vitamin E. Flaxseed oil supplementation increased the n-3 fatty acid concentration and oxidation products in the milk, while vitamin E supplementation increased milk’s antioxidant capacity. ABSTRACT: This study aimed to evaluate the effects of the supplementation of flaxseed oil and/or vitamin E on dry matter (DM) and nutrient digestibility, milk composition, fatty acid composition, and antioxidant capacity in buffalo milk. Four crossbred female dairy water buffaloes (97 ± 22 days in milk; 6.57 ± 2.2 kg of milk/day, mean ± SD) were distributed in a 4 × 4 Latin square design, with a 2 × 2 factorial arrangement (with or without flaxseed oil at 25 g/kg dry matter; with or without vitamin E at 375 IU/kg dry matter). The experimental period was divided into four periods of 21 days each (16 days for adaptation; five days for data collection). There were four treatments: control diet (no flaxseed oil and no added vitamin E); flaxseed oil diet (flaxseed oil at 25 g/kg DM); vitamin E diet (vitamin E at 375 IU/kg DM), and a combination of both flaxseed oil and vitamin E. The animals were fed total mixed ratios. For all response variables, there was no interaction between flaxseed oil and vitamin E. Flaxseed oil supplementation reduced neutral detergent fiber (NDF) and acid detergent fiber (ADF) apparent total tract digestibility, increased the n-3 fatty acid concentration in milk approximately three-fold while reducing the n-6/n-3 ratio from 9.3:1 to 2.4:1. Vitamin E supplementation increased NDF apparent total tract digestibility and milk total antioxidant capacity. Although there was no interaction between the treatments; flaxseed oil supplementation in lactating buffaloes increased polyunsaturated fatty acid, while vitamin E supplementation increased antioxidant capacity and decreased oxidation products.