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Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon
The cell wall of wild-type (WT) Mycobacterium tuberculosis (Mtb), an etiologic agent of tuberculosis (TB) and a Mtb strain disrupted in a 13-gene operon mce1 (Δmce1) varies by more than 400 lipid species. Here, we examined Mtb lipid-induced response in murine macrophage, as well as in human T-cell s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461851/ https://www.ncbi.nlm.nih.gov/pubmed/32973761 http://dx.doi.org/10.3389/fimmu.2020.01848 |
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author | Petrilli, Jéssica D. Müller, Igor Araújo, Luana E. Cardoso, Thiago M. Carvalho, Lucas P. Barros, Bruna C. Teixeira, Maurício Arruda, Sérgio Riley, Lee W. Queiroz, Adriano |
author_facet | Petrilli, Jéssica D. Müller, Igor Araújo, Luana E. Cardoso, Thiago M. Carvalho, Lucas P. Barros, Bruna C. Teixeira, Maurício Arruda, Sérgio Riley, Lee W. Queiroz, Adriano |
author_sort | Petrilli, Jéssica D. |
collection | PubMed |
description | The cell wall of wild-type (WT) Mycobacterium tuberculosis (Mtb), an etiologic agent of tuberculosis (TB) and a Mtb strain disrupted in a 13-gene operon mce1 (Δmce1) varies by more than 400 lipid species. Here, we examined Mtb lipid-induced response in murine macrophage, as well as in human T-cell subpopulations in order to gain an insight into how changes in cell wall lipid composition may modulate host immune response. Relative to WT Mtb cell wall lipids, the non-polar lipid extracts from Δmce1 enhanced the mRNA expression of lipid-sense nuclear receptors TR4 and PPAR-γ and dampened the macrophage expression of genes encoding TNF-α, IL-6, and IL-1β. Relative to untreated control, WT lipid-pre-stimulated macrophages from healthy individuals induced a higher level of CD4(−)CD8(−) double negative T-cells (DN T-cells) producing TNF-α. Conversely, compared to WT, stimulation with Δmce1 lipids induced higher mean fluorescence intensity (MFI) in IL-10-producing DN T cells. Mononuclear cells from TB patients stimulated with WT Mtb lipids induced an increased production of TNF-α by CD8(+) lymphocytes. Taken together, these observations suggest that changes in mce1 operon expression during a course of infection may serve as a strategy by Mtb to evade the host pro-inflammatory responses. |
format | Online Article Text |
id | pubmed-7461851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74618512020-09-23 Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon Petrilli, Jéssica D. Müller, Igor Araújo, Luana E. Cardoso, Thiago M. Carvalho, Lucas P. Barros, Bruna C. Teixeira, Maurício Arruda, Sérgio Riley, Lee W. Queiroz, Adriano Front Immunol Immunology The cell wall of wild-type (WT) Mycobacterium tuberculosis (Mtb), an etiologic agent of tuberculosis (TB) and a Mtb strain disrupted in a 13-gene operon mce1 (Δmce1) varies by more than 400 lipid species. Here, we examined Mtb lipid-induced response in murine macrophage, as well as in human T-cell subpopulations in order to gain an insight into how changes in cell wall lipid composition may modulate host immune response. Relative to WT Mtb cell wall lipids, the non-polar lipid extracts from Δmce1 enhanced the mRNA expression of lipid-sense nuclear receptors TR4 and PPAR-γ and dampened the macrophage expression of genes encoding TNF-α, IL-6, and IL-1β. Relative to untreated control, WT lipid-pre-stimulated macrophages from healthy individuals induced a higher level of CD4(−)CD8(−) double negative T-cells (DN T-cells) producing TNF-α. Conversely, compared to WT, stimulation with Δmce1 lipids induced higher mean fluorescence intensity (MFI) in IL-10-producing DN T cells. Mononuclear cells from TB patients stimulated with WT Mtb lipids induced an increased production of TNF-α by CD8(+) lymphocytes. Taken together, these observations suggest that changes in mce1 operon expression during a course of infection may serve as a strategy by Mtb to evade the host pro-inflammatory responses. Frontiers Media S.A. 2020-08-18 /pmc/articles/PMC7461851/ /pubmed/32973761 http://dx.doi.org/10.3389/fimmu.2020.01848 Text en Copyright © 2020 Petrilli, Müller, Araújo, Cardoso, Carvalho, Barros, Teixeira, Arruda, Riley and Queiroz. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Petrilli, Jéssica D. Müller, Igor Araújo, Luana E. Cardoso, Thiago M. Carvalho, Lucas P. Barros, Bruna C. Teixeira, Maurício Arruda, Sérgio Riley, Lee W. Queiroz, Adriano Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon |
title | Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon |
title_full | Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon |
title_fullStr | Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon |
title_full_unstemmed | Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon |
title_short | Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon |
title_sort | differential host pro-inflammatory response to mycobacterial cell wall lipids regulated by the mce1 operon |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7461851/ https://www.ncbi.nlm.nih.gov/pubmed/32973761 http://dx.doi.org/10.3389/fimmu.2020.01848 |
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