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Cholera Toxin Subunit B for Sensitive and Rapid Determination of Exosomes by Gel Filtration

We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-f...

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Detalles Bibliográficos
Autores principales: Sapoń, Karolina, Maziarz, Dominika, Janas, Teresa, Sikorski, Aleksander F., Janas, Tadeusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464461/
https://www.ncbi.nlm.nih.gov/pubmed/32751790
http://dx.doi.org/10.3390/membranes10080172