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In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit

The fruit of Ziziphus jujuba, commonly called jujube, has long been consumed for its health benefits. The aim of this study was to examine the protective effect of dietary supplementation of enzymatically hydrolyzed jujube against lung inflammation in mice. The macerated flesh of jujube was extracte...

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Autores principales: Kim, Yoonsu, Oh, Jisun, Jang, Chan Ho, Lim, Ji Sun, Lee, Jeong Soon, Kim, Jong-Sang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466189/
https://www.ncbi.nlm.nih.gov/pubmed/32752184
http://dx.doi.org/10.3390/foods9081033
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author Kim, Yoonsu
Oh, Jisun
Jang, Chan Ho
Lim, Ji Sun
Lee, Jeong Soon
Kim, Jong-Sang
author_facet Kim, Yoonsu
Oh, Jisun
Jang, Chan Ho
Lim, Ji Sun
Lee, Jeong Soon
Kim, Jong-Sang
author_sort Kim, Yoonsu
collection PubMed
description The fruit of Ziziphus jujuba, commonly called jujube, has long been consumed for its health benefits. The aim of this study was to examine the protective effect of dietary supplementation of enzymatically hydrolyzed jujube against lung inflammation in mice. The macerated flesh of jujube was extracted with aqueous ethanol before and after Viscozyme treatment. The extract of enzyme-treated jujube, called herein hydrolyzed jujube extract (HJE), contained higher levels of quercetin, total phenolics, and flavonoids, and exhibited more effective radical-scavenging abilities in comparison to non-hydrolyzed jujube extract (NHJE). HJE treatment decreased production of inflammation-associated molecules, including nitric oxide and pro-inflammatory cytokines from activated Raw 264.7 or differentiated THP-1 cells. HJE treatment also reduced expression of nuclear factor-κB and its downstream proteins in A549 human lung epithelial cells. Moreover, oral supplementation of 1.5 g of HJE per kg of body weight (BW) attenuated histological lung damage, decreased plasma cytokines, and inhibited expression of inflammatory proteins and oxidative stress mediators in the lungs of mice exposed to benzo(a)pyrene at 50 mg/kg BW. Expression levels of antioxidant and cytoprotective factors, such as nuclear factor erythroid-derived 2-related factor 2 and heme oxygenase-1, were increased in lung and liver tissues from mice treated with HJE, compared to mice fed NHJE. These findings indicate that dietary HJE can reduce benzo(a)pyrene-induced lung inflammation by inhibiting cytokine release from macrophages and promoting antioxidant defenses in vivo.
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spelling pubmed-74661892020-09-14 In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit Kim, Yoonsu Oh, Jisun Jang, Chan Ho Lim, Ji Sun Lee, Jeong Soon Kim, Jong-Sang Foods Article The fruit of Ziziphus jujuba, commonly called jujube, has long been consumed for its health benefits. The aim of this study was to examine the protective effect of dietary supplementation of enzymatically hydrolyzed jujube against lung inflammation in mice. The macerated flesh of jujube was extracted with aqueous ethanol before and after Viscozyme treatment. The extract of enzyme-treated jujube, called herein hydrolyzed jujube extract (HJE), contained higher levels of quercetin, total phenolics, and flavonoids, and exhibited more effective radical-scavenging abilities in comparison to non-hydrolyzed jujube extract (NHJE). HJE treatment decreased production of inflammation-associated molecules, including nitric oxide and pro-inflammatory cytokines from activated Raw 264.7 or differentiated THP-1 cells. HJE treatment also reduced expression of nuclear factor-κB and its downstream proteins in A549 human lung epithelial cells. Moreover, oral supplementation of 1.5 g of HJE per kg of body weight (BW) attenuated histological lung damage, decreased plasma cytokines, and inhibited expression of inflammatory proteins and oxidative stress mediators in the lungs of mice exposed to benzo(a)pyrene at 50 mg/kg BW. Expression levels of antioxidant and cytoprotective factors, such as nuclear factor erythroid-derived 2-related factor 2 and heme oxygenase-1, were increased in lung and liver tissues from mice treated with HJE, compared to mice fed NHJE. These findings indicate that dietary HJE can reduce benzo(a)pyrene-induced lung inflammation by inhibiting cytokine release from macrophages and promoting antioxidant defenses in vivo. MDPI 2020-08-01 /pmc/articles/PMC7466189/ /pubmed/32752184 http://dx.doi.org/10.3390/foods9081033 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yoonsu
Oh, Jisun
Jang, Chan Ho
Lim, Ji Sun
Lee, Jeong Soon
Kim, Jong-Sang
In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit
title In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit
title_full In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit
title_fullStr In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit
title_full_unstemmed In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit
title_short In Vivo Anti-Inflammatory Potential of Viscozyme(®)-Treated Jujube Fruit
title_sort in vivo anti-inflammatory potential of viscozyme(®)-treated jujube fruit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466189/
https://www.ncbi.nlm.nih.gov/pubmed/32752184
http://dx.doi.org/10.3390/foods9081033
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