Cargando…
Microtubule instability driven by longitudinal and lateral strain propagation
Tubulin dimers associate longitudinally and laterally to form metastable microtubules (MTs). MT disassembly is preceded by subtle structural changes in tubulin fueled by GTP hydrolysis. These changes render the MT lattice unstable, but it is unclear exactly how they affect lattice energetics and str...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467311/ https://www.ncbi.nlm.nih.gov/pubmed/32877399 http://dx.doi.org/10.1371/journal.pcbi.1008132 |
_version_ | 1783577985509490688 |
---|---|
author | Igaev, Maxim Grubmüller, Helmut |
author_facet | Igaev, Maxim Grubmüller, Helmut |
author_sort | Igaev, Maxim |
collection | PubMed |
description | Tubulin dimers associate longitudinally and laterally to form metastable microtubules (MTs). MT disassembly is preceded by subtle structural changes in tubulin fueled by GTP hydrolysis. These changes render the MT lattice unstable, but it is unclear exactly how they affect lattice energetics and strain. We performed long-time atomistic simulations to interrogate the impacts of GTP hydrolysis on tubulin lattice conformation, lateral inter-dimer interactions, and (non-)local lateral coordination of dimer motions. The simulations suggest that most of the hydrolysis energy is stored in the lattice in the form of longitudinal strain. While not significantly affecting lateral bond stability, the stored elastic energy results in more strongly confined and correlated dynamics of GDP-tubulins, thereby entropically destabilizing the MT lattice. |
format | Online Article Text |
id | pubmed-7467311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-74673112020-09-11 Microtubule instability driven by longitudinal and lateral strain propagation Igaev, Maxim Grubmüller, Helmut PLoS Comput Biol Research Article Tubulin dimers associate longitudinally and laterally to form metastable microtubules (MTs). MT disassembly is preceded by subtle structural changes in tubulin fueled by GTP hydrolysis. These changes render the MT lattice unstable, but it is unclear exactly how they affect lattice energetics and strain. We performed long-time atomistic simulations to interrogate the impacts of GTP hydrolysis on tubulin lattice conformation, lateral inter-dimer interactions, and (non-)local lateral coordination of dimer motions. The simulations suggest that most of the hydrolysis energy is stored in the lattice in the form of longitudinal strain. While not significantly affecting lateral bond stability, the stored elastic energy results in more strongly confined and correlated dynamics of GDP-tubulins, thereby entropically destabilizing the MT lattice. Public Library of Science 2020-09-02 /pmc/articles/PMC7467311/ /pubmed/32877399 http://dx.doi.org/10.1371/journal.pcbi.1008132 Text en © 2020 Igaev, Grubmüller http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Igaev, Maxim Grubmüller, Helmut Microtubule instability driven by longitudinal and lateral strain propagation |
title | Microtubule instability driven by longitudinal and lateral strain propagation |
title_full | Microtubule instability driven by longitudinal and lateral strain propagation |
title_fullStr | Microtubule instability driven by longitudinal and lateral strain propagation |
title_full_unstemmed | Microtubule instability driven by longitudinal and lateral strain propagation |
title_short | Microtubule instability driven by longitudinal and lateral strain propagation |
title_sort | microtubule instability driven by longitudinal and lateral strain propagation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467311/ https://www.ncbi.nlm.nih.gov/pubmed/32877399 http://dx.doi.org/10.1371/journal.pcbi.1008132 |
work_keys_str_mv | AT igaevmaxim microtubuleinstabilitydrivenbylongitudinalandlateralstrainpropagation AT grubmullerhelmut microtubuleinstabilitydrivenbylongitudinalandlateralstrainpropagation |