A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase

Deoxyribonucleoside triphosphates (dNTPs) are vital for the biosynthesis and repair of DNA. Their cellular concentration peaks during the S phase of the cell cycle. In non-proliferating cells, dNTP concentrations are low, making their reliable quantification from tissue samples of heterogeneous cell...

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Detalles Bibliográficos
Autores principales: Purhonen, Janne, Banerjee, Rishi, McDonald, Allison E, Fellman, Vineta, Kallijärvi, Jukka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470940/
https://www.ncbi.nlm.nih.gov/pubmed/32573728
http://dx.doi.org/10.1093/nar/gkaa516

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470940/
https://www.ncbi.nlm.nih.gov/pubmed/32573728
http://dx.doi.org/10.1093/nar/gkaa516

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