Cargando…

A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye and inhibitor-resistant high-fidelity DNA polymerase

Deoxyribonucleoside triphosphates (dNTPs) are vital for the biosynthesis and repair of DNA. Their cellular concentration peaks during the S phase of the cell cycle. In non-proliferating cells, dNTP concentrations are low, making their reliable quantification from tissue samples of heterogeneous cell...

Descripción completa

Detalles Bibliográficos
Autores principales:	Purhonen, Janne, Banerjee, Rishi, McDonald, Allison E, Fellman, Vineta, Kallijärvi, Jukka
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470940/ https://www.ncbi.nlm.nih.gov/pubmed/32573728 http://dx.doi.org/10.1093/nar/gkaa516

Ejemplares similares

Isocratic HPLC analysis for the simultaneous determination of dNTPs, rNTPs and ADP in biological samples
por: Ranjbarian, Farahnaz, et al.
Publicado: (2021)

Mechanisms of telomerase inhibition by oxidized and therapeutic dNTPs
por: Sanford, Samantha L., et al.
Publicado: (2020)

Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases
por: Pospíšil, Šimon, et al.
Publicado: (2022)

A Critical Balance: dNTPs and the Maintenance of Genome Stability
por: Pai, Chen-Chun, et al.
Publicado: (2017)

Mitochondrial complex III deficiency drives c-MYC overexpression and illicit cell cycle entry leading to senescence and segmental progeria
por: Purhonen, Janne, et al.
Publicado: (2023)

Enzymatic assay for UDP-GlcNAc and its application in the parallel assessment of substrate availability and protein O-GlcNAcylation
por: Sunden, Marc, et al.
Publicado: (2023)

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
por: Eischeid, Anne C
Publicado: (2011)

Exploration of factors driving incorporation of unnatural dNTPS into DNA by Klenow fragment (DNA polymerase I) and DNA polymerase α
por: Kincaid, Kristi, et al.
Publicado: (2005)

MYH9 binds to dNTPs via deoxyribose moiety and plays an important role in DNA synthesis
por: Nangia-Makker, Pratima, et al.
Publicado: (2022)

Oxidized dNTPs and the OGG1 and MUTYH DNA glycosylases combine to induce CAG/CTG repeat instability
por: Cilli, Piera, et al.
Publicado: (2016)

Rapamycin – “One size does not fit all”
por: Kallijärvi, Jukka, et al.
Publicado: (2019)

Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
por: Mao, Fei, et al.
Publicado: (2007)

5′CAG and 5′CTG Repeats Create Differential Impediment to the Progression of a Minimal Reconstituted T4 Replisome Depending on the Concentration of dNTPs
por: Delagoutte, Emmanuelle, et al.
Publicado: (2011)

Solid-Phase Primer Elongation Using Biotinylated dNTPs for the Detection of a Single Nucleotide Polymorphism from a Fingerprick Blood Sample
por: Jauset-Rubio, Miriam, et al.
Publicado: (2021)

Application of EvaGreen for the assessment of Listeria monocytogenes АТСС 13932 cell viability using flow cytometry
por: Kotenkova, Elena, et al.
Publicado: (2019)

A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice
por: Purhonen, Janne, et al.
Publicado: (2020)

MYC—an emerging player in mitochondrial diseases
por: Purhonen, Janne, et al.
Publicado: (2023)

Ketogenic diet attenuates hepatopathy in mouse model of respiratory chain complex III deficiency caused by a Bcs1l mutation
por: Purhonen, Janne, et al.
Publicado: (2017)

COX7A2L/SCAFI and Pre-Complex III Modify Respiratory Chain Supercomplex Formation in Different Mouse Strains with a Bcs1l Mutation
por: Davoudi, Mina, et al.
Publicado: (2016)

A Simultaneous Analytical Method for Duplex Identification of Porcine and Horse in the Meat Products by EvaGreen based Real-time PCR
por: Sakalar, Ergün, et al.
Publicado: (2015)

Effect of High-Carbohydrate Diet on Plasma Metabolome in Mice with Mitochondrial Respiratory Chain Complex III Deficiency
por: Rajendran, Jayasimman, et al.
Publicado: (2016)

Alternative oxidase‐mediated respiration prevents lethal mitochondrial cardiomyopathy
por: Rajendran, Jayasimman, et al.
Publicado: (2018)

Single-tube multiplex real-time PCR with EvaGreen and high-resolution melting analysis for diagnosis of α(0)-thalassemia--(SEA),--(THAI), and--(CR) type deletions
por: Ruengdit, Chedtapak, et al.
Publicado: (2023)

Plasmid-based lacZα assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
por: Jozwiakowski, Stanislaw K., et al.
Publicado: (2009)

Respiratory chain complex III deficiency due to mutated BCS1L: a novel phenotype with encephalomyopathy, partially phenocopied in a Bcs1l mutant mouse model
por: Tegelberg, Saara, et al.
Publicado: (2017)

High-resolution mapping of DNA polymerase fidelity using nucleotide imbalances and next-generation sequencing
por: de Paz, Alexandra M, et al.
Publicado: (2018)

Spatiotemporally controlled generation of NTPs for single-molecule studies
por: Sabantsev, Anton, et al.
Publicado: (2022)

Epidemiological investigation of feline infectious peritonitis in cats living in Harbin, Northeast China from 2017 to 2019 using a combination of an EvaGreen-based real-time RT-PCR and serum chemistry assays
por: Guan, Xueting, et al.
Publicado: (2020)

Synthesis of α-l-Threofuranosyl Nucleoside Triphosphates (tNTPs)
por: Zou, Keyong, et al.
Publicado: (2005)

The presence of rNTPs decreases the speed of mitochondrial DNA replication
por: Forslund, Josefin M. E., et al.
Publicado: (2018)

A Versatile qPCR for Diagnosis of Leporid Gammaherpesvirus 5 Using Evagreen(®) or Taqman(®) Technologies
por: Abade dos Santos, Fábio A., et al.
Publicado: (2021)

Redirecting splicing with bifunctional oligonucleotides
por: Brosseau, Jean-Philippe, et al.
Publicado: (2014)

qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites
por: Dabrowska, Magdalena, et al.
Publicado: (2018)

Retooling National TB Control Programmes (NTPs) with New Diagnostics: The NTP Perspective
por: van Kampen, Sanne C., et al.
Publicado: (2010)

Human and mouse oligonucleotide-based array CGH
por: van den IJssel, Paul, et al.
Publicado: (2005)

Assessing incomplete deprotection of microarray oligonucleotides in situ
por: Dressman, Holly K., et al.
Publicado: (2006)

Genome-wide selection of unique and valid oligonucleotides
por: Hyyrö, Heikki, et al.
Publicado: (2005)

Rapid oligonucleotide-templated fluorogenic tetrazine ligations
por: Šečkutė, Jolita, et al.
Publicado: (2013)

Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication
por: Egeland, Ryan D., et al.
Publicado: (2005)

Evaluating oligonucleotide properties for DNA microarray probe design
por: Xia, Xiao-Qin, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_u81ebil6peubg6si117t2orvqi