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Assessment of Inter-Laboratory Variation in the Characterization and Analysis of the Mucosal Microbiota in Crohn’s Disease and Ulcerative Colitis

BACKGROUND: In studies evaluating the microbiome, numerous factors can contribute to technical variability. These factors include DNA extraction methodology, sequencing protocols, and data analysis strategies. We sought to evaluate the impact these factors have on the results obtained when the seque...

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Detalles Bibliográficos
Autores principales: Szamosi, Jake C., Forbes, Jessica D., Copeland, Julia K., Knox, Natalie C., Shekarriz, Shahrokh, Rossi, Laura, Graham, Morag, Bonner, Christine, Guttman, David S., Van Domselaar, Gary, Surette, Michael G., Bernstein, Charles N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472644/
https://www.ncbi.nlm.nih.gov/pubmed/32973734
http://dx.doi.org/10.3389/fmicb.2020.02028