Cargando…

CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis

CD103 is considered as a surface marker for the resident immune cells. However, little is known about the intrinsic function of CD103 in infection and inflammation. In this study, we found that CD103 was highly expressed in CD4(+)T cells of the gastric mucosa from patients with H. pylori-positive ga...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Peiyu, Ming, Siqi, Lao, Juanfeng, Li, Chunna, Wang, Hongli, Xiong, Liya, Zhang, Shunxian, Liang, Zibin, Niu, Xiaoli, Deng, Simei, Geng, Lanlan, Wu, Minhao, Wu, Yongjian, Gong, Sitang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472738/
https://www.ncbi.nlm.nih.gov/pubmed/32974219
http://dx.doi.org/10.3389/fcimb.2020.00436
_version_ 1783579043288842240
author Chen, Peiyu
Ming, Siqi
Lao, Juanfeng
Li, Chunna
Wang, Hongli
Xiong, Liya
Zhang, Shunxian
Liang, Zibin
Niu, Xiaoli
Deng, Simei
Geng, Lanlan
Wu, Minhao
Wu, Yongjian
Gong, Sitang
author_facet Chen, Peiyu
Ming, Siqi
Lao, Juanfeng
Li, Chunna
Wang, Hongli
Xiong, Liya
Zhang, Shunxian
Liang, Zibin
Niu, Xiaoli
Deng, Simei
Geng, Lanlan
Wu, Minhao
Wu, Yongjian
Gong, Sitang
author_sort Chen, Peiyu
collection PubMed
description CD103 is considered as a surface marker for the resident immune cells. However, little is known about the intrinsic function of CD103 in infection and inflammation. In this study, we found that CD103 was highly expressed in CD4(+)T cells of the gastric mucosa from patients with H. pylori-positive gastritis. Mucosal resident CD103(+)CD4(+)T cells exhibited an increase in the CD45RO(+)CCR7(−) effector memory phenotype and high expression of the chemokine receptors CXCR3 and CCR9 compared with those in CD103(−)CD4(+)T cells. An In vitro coculture study demonstrated that H. pylori-specific antigen CagA/VacA-primed dendritic cells (DCs) induced proliferation and IFN-γ, TNF as well as IL-17 production by CD103(+)CD4(+)T cells from patients with H. pylori-positive gastritis, while blocking CD103 with a neutralizing antibody reduced proliferation and IFN-γ, TNF, and IL-17 production by CD103(+)CD4(+)T cells cocultured with DCs. Moreover, immunoprecipitation revealed that CD103 interacted with TCR α/β and CD3ζ, and activation of CD103 enhanced the phosphorylation of ZAP70 induced by the TCR signal. Finally, increased T-bet and Blimp1 levels were also observed in CD103(+)CD4(+)T cells, and activating CD103 increased T-bet and Blimp1 expression in CD4(+)T cells. Our results explored the intrinsic function of CD103 in gastric T cells from patients with H. pylori-positive gastritis, which may provide a therapeutic target for the treatment of gastritis.
format Online
Article
Text
id pubmed-7472738
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-74727382020-09-23 CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis Chen, Peiyu Ming, Siqi Lao, Juanfeng Li, Chunna Wang, Hongli Xiong, Liya Zhang, Shunxian Liang, Zibin Niu, Xiaoli Deng, Simei Geng, Lanlan Wu, Minhao Wu, Yongjian Gong, Sitang Front Cell Infect Microbiol Cellular and Infection Microbiology CD103 is considered as a surface marker for the resident immune cells. However, little is known about the intrinsic function of CD103 in infection and inflammation. In this study, we found that CD103 was highly expressed in CD4(+)T cells of the gastric mucosa from patients with H. pylori-positive gastritis. Mucosal resident CD103(+)CD4(+)T cells exhibited an increase in the CD45RO(+)CCR7(−) effector memory phenotype and high expression of the chemokine receptors CXCR3 and CCR9 compared with those in CD103(−)CD4(+)T cells. An In vitro coculture study demonstrated that H. pylori-specific antigen CagA/VacA-primed dendritic cells (DCs) induced proliferation and IFN-γ, TNF as well as IL-17 production by CD103(+)CD4(+)T cells from patients with H. pylori-positive gastritis, while blocking CD103 with a neutralizing antibody reduced proliferation and IFN-γ, TNF, and IL-17 production by CD103(+)CD4(+)T cells cocultured with DCs. Moreover, immunoprecipitation revealed that CD103 interacted with TCR α/β and CD3ζ, and activation of CD103 enhanced the phosphorylation of ZAP70 induced by the TCR signal. Finally, increased T-bet and Blimp1 levels were also observed in CD103(+)CD4(+)T cells, and activating CD103 increased T-bet and Blimp1 expression in CD4(+)T cells. Our results explored the intrinsic function of CD103 in gastric T cells from patients with H. pylori-positive gastritis, which may provide a therapeutic target for the treatment of gastritis. Frontiers Media S.A. 2020-08-21 /pmc/articles/PMC7472738/ /pubmed/32974219 http://dx.doi.org/10.3389/fcimb.2020.00436 Text en Copyright © 2020 Chen, Ming, Lao, Li, Wang, Xiong, Zhang, Liang, Niu, Deng, Geng, Wu, Wu and Gong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Chen, Peiyu
Ming, Siqi
Lao, Juanfeng
Li, Chunna
Wang, Hongli
Xiong, Liya
Zhang, Shunxian
Liang, Zibin
Niu, Xiaoli
Deng, Simei
Geng, Lanlan
Wu, Minhao
Wu, Yongjian
Gong, Sitang
CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
title CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
title_full CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
title_fullStr CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
title_full_unstemmed CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
title_short CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
title_sort cd103 promotes the pro-inflammatory response of gastric resident cd4(+) t cell in helicobacter pylori-positive gastritis
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472738/
https://www.ncbi.nlm.nih.gov/pubmed/32974219
http://dx.doi.org/10.3389/fcimb.2020.00436
work_keys_str_mv AT chenpeiyu cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT mingsiqi cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT laojuanfeng cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT lichunna cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT wanghongli cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT xiongliya cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT zhangshunxian cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT liangzibin cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT niuxiaoli cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT dengsimei cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT genglanlan cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT wuminhao cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT wuyongjian cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis
AT gongsitang cd103promotestheproinflammatoryresponseofgastricresidentcd4tcellinhelicobacterpyloripositivegastritis