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CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis
CD103 is considered as a surface marker for the resident immune cells. However, little is known about the intrinsic function of CD103 in infection and inflammation. In this study, we found that CD103 was highly expressed in CD4(+)T cells of the gastric mucosa from patients with H. pylori-positive ga...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472738/ https://www.ncbi.nlm.nih.gov/pubmed/32974219 http://dx.doi.org/10.3389/fcimb.2020.00436 |
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author | Chen, Peiyu Ming, Siqi Lao, Juanfeng Li, Chunna Wang, Hongli Xiong, Liya Zhang, Shunxian Liang, Zibin Niu, Xiaoli Deng, Simei Geng, Lanlan Wu, Minhao Wu, Yongjian Gong, Sitang |
author_facet | Chen, Peiyu Ming, Siqi Lao, Juanfeng Li, Chunna Wang, Hongli Xiong, Liya Zhang, Shunxian Liang, Zibin Niu, Xiaoli Deng, Simei Geng, Lanlan Wu, Minhao Wu, Yongjian Gong, Sitang |
author_sort | Chen, Peiyu |
collection | PubMed |
description | CD103 is considered as a surface marker for the resident immune cells. However, little is known about the intrinsic function of CD103 in infection and inflammation. In this study, we found that CD103 was highly expressed in CD4(+)T cells of the gastric mucosa from patients with H. pylori-positive gastritis. Mucosal resident CD103(+)CD4(+)T cells exhibited an increase in the CD45RO(+)CCR7(−) effector memory phenotype and high expression of the chemokine receptors CXCR3 and CCR9 compared with those in CD103(−)CD4(+)T cells. An In vitro coculture study demonstrated that H. pylori-specific antigen CagA/VacA-primed dendritic cells (DCs) induced proliferation and IFN-γ, TNF as well as IL-17 production by CD103(+)CD4(+)T cells from patients with H. pylori-positive gastritis, while blocking CD103 with a neutralizing antibody reduced proliferation and IFN-γ, TNF, and IL-17 production by CD103(+)CD4(+)T cells cocultured with DCs. Moreover, immunoprecipitation revealed that CD103 interacted with TCR α/β and CD3ζ, and activation of CD103 enhanced the phosphorylation of ZAP70 induced by the TCR signal. Finally, increased T-bet and Blimp1 levels were also observed in CD103(+)CD4(+)T cells, and activating CD103 increased T-bet and Blimp1 expression in CD4(+)T cells. Our results explored the intrinsic function of CD103 in gastric T cells from patients with H. pylori-positive gastritis, which may provide a therapeutic target for the treatment of gastritis. |
format | Online Article Text |
id | pubmed-7472738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74727382020-09-23 CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis Chen, Peiyu Ming, Siqi Lao, Juanfeng Li, Chunna Wang, Hongli Xiong, Liya Zhang, Shunxian Liang, Zibin Niu, Xiaoli Deng, Simei Geng, Lanlan Wu, Minhao Wu, Yongjian Gong, Sitang Front Cell Infect Microbiol Cellular and Infection Microbiology CD103 is considered as a surface marker for the resident immune cells. However, little is known about the intrinsic function of CD103 in infection and inflammation. In this study, we found that CD103 was highly expressed in CD4(+)T cells of the gastric mucosa from patients with H. pylori-positive gastritis. Mucosal resident CD103(+)CD4(+)T cells exhibited an increase in the CD45RO(+)CCR7(−) effector memory phenotype and high expression of the chemokine receptors CXCR3 and CCR9 compared with those in CD103(−)CD4(+)T cells. An In vitro coculture study demonstrated that H. pylori-specific antigen CagA/VacA-primed dendritic cells (DCs) induced proliferation and IFN-γ, TNF as well as IL-17 production by CD103(+)CD4(+)T cells from patients with H. pylori-positive gastritis, while blocking CD103 with a neutralizing antibody reduced proliferation and IFN-γ, TNF, and IL-17 production by CD103(+)CD4(+)T cells cocultured with DCs. Moreover, immunoprecipitation revealed that CD103 interacted with TCR α/β and CD3ζ, and activation of CD103 enhanced the phosphorylation of ZAP70 induced by the TCR signal. Finally, increased T-bet and Blimp1 levels were also observed in CD103(+)CD4(+)T cells, and activating CD103 increased T-bet and Blimp1 expression in CD4(+)T cells. Our results explored the intrinsic function of CD103 in gastric T cells from patients with H. pylori-positive gastritis, which may provide a therapeutic target for the treatment of gastritis. Frontiers Media S.A. 2020-08-21 /pmc/articles/PMC7472738/ /pubmed/32974219 http://dx.doi.org/10.3389/fcimb.2020.00436 Text en Copyright © 2020 Chen, Ming, Lao, Li, Wang, Xiong, Zhang, Liang, Niu, Deng, Geng, Wu, Wu and Gong. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Chen, Peiyu Ming, Siqi Lao, Juanfeng Li, Chunna Wang, Hongli Xiong, Liya Zhang, Shunxian Liang, Zibin Niu, Xiaoli Deng, Simei Geng, Lanlan Wu, Minhao Wu, Yongjian Gong, Sitang CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis |
title | CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis |
title_full | CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis |
title_fullStr | CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis |
title_full_unstemmed | CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis |
title_short | CD103 Promotes the Pro-inflammatory Response of Gastric Resident CD4(+) T Cell in Helicobacter pylori-Positive Gastritis |
title_sort | cd103 promotes the pro-inflammatory response of gastric resident cd4(+) t cell in helicobacter pylori-positive gastritis |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472738/ https://www.ncbi.nlm.nih.gov/pubmed/32974219 http://dx.doi.org/10.3389/fcimb.2020.00436 |
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