Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos

CRISPR/Cas9 genome editing has revolutionized functional genomics in vertebrates. However, CRISPR/Cas9 edited F(0) animals too often demonstrate variable phenotypic penetrance due to the mosaic nature of editing outcomes after double strand break (DSB) repair. Even with high efficiency levels of gen...

Descripción completa

Detalles Bibliográficos
Autores principales: Naert, Thomas, Tulkens, Dieter, Edwards, Nicole A., Carron, Marjolein, Shaidani, Nikko-Ideen, Wlizla, Marcin, Boel, Annekatrien, Demuynck, Suzan, Horb, Marko E., Coucke, Paul, Willaert, Andy, Zorn, Aaron M., Vleminckx, Kris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473854/
https://www.ncbi.nlm.nih.gov/pubmed/32887910
http://dx.doi.org/10.1038/s41598-020-71412-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473854/
https://www.ncbi.nlm.nih.gov/pubmed/32887910
http://dx.doi.org/10.1038/s41598-020-71412-0

Ejemplares similares