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Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy

DNA intermediate structures are formed in all major pathways of DNA metabolism. Transmission electron microscopy (TEM) is a tool of choice to study their choreography and has led to major advances in the understanding of these mechanisms, particularly those of homologous recombination (HR) and repli...

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Detalles Bibliográficos
Autores principales: Benureau, Yann, Moreira Tavares, Eliana, Muhammad, Ali-Akbar, Baconnais, Sonia, Le Cam, Eric, Dupaigne, Pauline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474861/
https://www.ncbi.nlm.nih.gov/pubmed/32913896
http://dx.doi.org/10.1093/biomethods/bpaa012