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Isolation and next generation sequencing of archival formalin‐fixed DNA

DNA from archived organs is presumed unsuitable for genomic studies because of excessive formalin‐fixation. As next generation sequencing (NGS) requires short DNA fragments, and Uracil‐N‐glycosylase (UNG) can be used to overcome deamination, there has been renewed interest in the possibility of geno...

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Detalles Bibliográficos
Autores principales: Alqahtani, Ahlam, Skelton, Andrew, Eley, Lorraine, Annavarapu, Srinivas, Henderson, Deborah J., Chaudhry, Bill
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476199/
https://www.ncbi.nlm.nih.gov/pubmed/32426881
http://dx.doi.org/10.1111/joa.13209