Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan
While the function of proteins and genes has been widely studied during vertebrate development, relatively little work has addressed the role of carbohydrates. Hyaluronan (HA), also known as hyaluronic acid, is an abundant carbohydrate in embryonic tissues and is the main structural component of the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476214/ https://www.ncbi.nlm.nih.gov/pubmed/32982773 http://dx.doi.org/10.3389/fphys.2020.00996 |
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author | Sánchez, Natalia González-Ramírez, María Constanza Contreras, Esteban G. Ubilla, Angélica Li, Jingjing Valencia, Anyeli Wilson, Andrés Green, Jeremy B. A. Tucker, Abigail S. Gaete, Marcia |
author_facet | Sánchez, Natalia González-Ramírez, María Constanza Contreras, Esteban G. Ubilla, Angélica Li, Jingjing Valencia, Anyeli Wilson, Andrés Green, Jeremy B. A. Tucker, Abigail S. Gaete, Marcia |
author_sort | Sánchez, Natalia |
collection | PubMed |
description | While the function of proteins and genes has been widely studied during vertebrate development, relatively little work has addressed the role of carbohydrates. Hyaluronan (HA), also known as hyaluronic acid, is an abundant carbohydrate in embryonic tissues and is the main structural component of the extracellular matrix of epithelial and mesenchymal cells. HA is able to absorb large quantities of water and can signal by binding to cell-surface receptors. During organ development and regeneration, HA has been shown to regulate cell proliferation, cell shape, and migration. Here, we have investigated the function of HA during molar tooth development in mice, in which, similar to humans, new molars sequentially bud off from a pre-existing molar. Using an ex vivo approach, we found that inhibiting HA synthesis in culture leads to a significant increase in proliferation and subsequent size of the developing molar, while the formation of sequential molars was inhibited. By cell shape analysis, we observed that inhibition of HA synthesis caused an elongation and reorientation of the major cell axes, indicating that disruption to cellular orientation and shape may underlie the observed phenotype. Lineage tracing demonstrated the retention of cells in the developing first molar (M1) at the expense of the generation of a second molar (M2). Our results highlight a novel role for HA in controlling proliferation, cell orientation, and migration in the developing tooth, impacting cellular decisions regarding tooth size and number. |
format | Online Article Text |
id | pubmed-7476214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74762142020-09-26 Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan Sánchez, Natalia González-Ramírez, María Constanza Contreras, Esteban G. Ubilla, Angélica Li, Jingjing Valencia, Anyeli Wilson, Andrés Green, Jeremy B. A. Tucker, Abigail S. Gaete, Marcia Front Physiol Physiology While the function of proteins and genes has been widely studied during vertebrate development, relatively little work has addressed the role of carbohydrates. Hyaluronan (HA), also known as hyaluronic acid, is an abundant carbohydrate in embryonic tissues and is the main structural component of the extracellular matrix of epithelial and mesenchymal cells. HA is able to absorb large quantities of water and can signal by binding to cell-surface receptors. During organ development and regeneration, HA has been shown to regulate cell proliferation, cell shape, and migration. Here, we have investigated the function of HA during molar tooth development in mice, in which, similar to humans, new molars sequentially bud off from a pre-existing molar. Using an ex vivo approach, we found that inhibiting HA synthesis in culture leads to a significant increase in proliferation and subsequent size of the developing molar, while the formation of sequential molars was inhibited. By cell shape analysis, we observed that inhibition of HA synthesis caused an elongation and reorientation of the major cell axes, indicating that disruption to cellular orientation and shape may underlie the observed phenotype. Lineage tracing demonstrated the retention of cells in the developing first molar (M1) at the expense of the generation of a second molar (M2). Our results highlight a novel role for HA in controlling proliferation, cell orientation, and migration in the developing tooth, impacting cellular decisions regarding tooth size and number. Frontiers Media S.A. 2020-08-24 /pmc/articles/PMC7476214/ /pubmed/32982773 http://dx.doi.org/10.3389/fphys.2020.00996 Text en Copyright © 2020 Sánchez, González-Ramírez, Contreras, Ubilla, Li, Valencia, Wilson, Green, Tucker and Gaete. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Sánchez, Natalia González-Ramírez, María Constanza Contreras, Esteban G. Ubilla, Angélica Li, Jingjing Valencia, Anyeli Wilson, Andrés Green, Jeremy B. A. Tucker, Abigail S. Gaete, Marcia Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan |
title | Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan |
title_full | Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan |
title_fullStr | Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan |
title_full_unstemmed | Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan |
title_short | Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan |
title_sort | balance between tooth size and tooth number is controlled by hyaluronan |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476214/ https://www.ncbi.nlm.nih.gov/pubmed/32982773 http://dx.doi.org/10.3389/fphys.2020.00996 |
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