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Optimized protocol for the preparation of single cells from cutaneous wounds for flow cytometric cell sorting and analysis of macrophages

The incidence of chronic, non-healing skin wounds is accelerating, largely due to the epidemic of obesity-related Type 2 diabetes. Abnormal inflammation in wounds contributes to delayed healing. During wound repair, blood monocytes are recruited into the wound bed where they differentiate into macro...

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Detalles Bibliográficos
Autores principales: Shakya, Sajina, Asosingh, Kewal, Mack, Judith A., Maytin, Edward V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476874/
https://www.ncbi.nlm.nih.gov/pubmed/32939347
http://dx.doi.org/10.1016/j.mex.2020.101027