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Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe

BACKGROUND: Facing the emergence of a new RNA virus, clinical laboratories are often helpless in the case of a shortage of reagents recommended by Reference Centres. OBJECTIVES: To compare five open one step RT-qPCR reagents to the SuperScript™ III Platinum™ One-Step qRT-PCR kit (Invitrogen) conside...

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Detalles Bibliográficos
Autores principales: Haddar, Cyrille, Verhoeven, Paul O., Bourlet, Thomas, Pozzetto, Bruno, Pillet, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476897/
https://www.ncbi.nlm.nih.gov/pubmed/33099260
http://dx.doi.org/10.1016/j.jcv.2020.104636