GFP fluorescence tagging alters dynamin-related protein 1 oligomerization dynamics and creates disassembly-refractory puncta to mediate mitochondrial fission

Green fluorescent protein (GFP)-tagging is the prevalent strategy to monitor protein dynamics in living cells. However, the consequences of appending the bulky GFP moiety to the protein of interest are rarely investigated. Here, using a powerful combination of quantitative fluorescence spectroscopic...

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Detalles Bibliográficos
Autores principales: Montecinos-Franjola, Felipe, Bauer, Brianna L., Mears, Jason A., Ramachandran, Rajesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479153/
https://www.ncbi.nlm.nih.gov/pubmed/32901052
http://dx.doi.org/10.1038/s41598-020-71655-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479153/
https://www.ncbi.nlm.nih.gov/pubmed/32901052
http://dx.doi.org/10.1038/s41598-020-71655-x

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