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Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function
Azithromycin (AZM) has been widely used as an antibacterial drug for many years. It has also been used to treat delayed gastric emptying. However, it exerts several side effects. We found that deglycosylated AZM (Deg-AZM or CP0119), an AZM metabolite, is a positively strong intestinal agonist that m...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479357/ https://www.ncbi.nlm.nih.gov/pubmed/32889431 http://dx.doi.org/10.1016/j.isci.2020.101464 |
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author | Zhong, Weilong Sun, Bo Ruan, Hao Yang, Guang Qian, Baoxin Cao, Hailong He, Lingfei Fan, Yunjing Roberts, Arthur G. Liu, Xiang Hu, Xuejiao Liang, Yuan Ye, Qing Yin, Tingting Wang, Bangmao Yang, Cheng Sun, Tao Zhou, Honggang |
author_facet | Zhong, Weilong Sun, Bo Ruan, Hao Yang, Guang Qian, Baoxin Cao, Hailong He, Lingfei Fan, Yunjing Roberts, Arthur G. Liu, Xiang Hu, Xuejiao Liang, Yuan Ye, Qing Yin, Tingting Wang, Bangmao Yang, Cheng Sun, Tao Zhou, Honggang |
author_sort | Zhong, Weilong |
collection | PubMed |
description | Azithromycin (AZM) has been widely used as an antibacterial drug for many years. It has also been used to treat delayed gastric emptying. However, it exerts several side effects. We found that deglycosylated AZM (Deg-AZM or CP0119), an AZM metabolite, is a positively strong intestinal agonist that may result in the intestinal mobility experienced by patients after AZM administration. We confirmed that Deg-AZM can function strongly on intestinal peristalsis and identified transgelin as its potential molecular target. Furthermore, our pharmacological studies showed that the binding of Deg-AZM to transgelin enhanced the contractility of intestinal smooth muscle cells by facilitating the assembly of actin filaments into tight bundles and stress fibers. Specifically, Deg-AZM promoted intestinal peristaltic activity in wild-type mice but not in transgelin (−/−) mice. Moreover, Deg-AZM did not exert antibacterial activity and did not disrupt intestinal flora. Thus, Deg-AZM may become a potential drug for slow-transit constipation treatment. |
format | Online Article Text |
id | pubmed-7479357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-74793572020-09-15 Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function Zhong, Weilong Sun, Bo Ruan, Hao Yang, Guang Qian, Baoxin Cao, Hailong He, Lingfei Fan, Yunjing Roberts, Arthur G. Liu, Xiang Hu, Xuejiao Liang, Yuan Ye, Qing Yin, Tingting Wang, Bangmao Yang, Cheng Sun, Tao Zhou, Honggang iScience Article Azithromycin (AZM) has been widely used as an antibacterial drug for many years. It has also been used to treat delayed gastric emptying. However, it exerts several side effects. We found that deglycosylated AZM (Deg-AZM or CP0119), an AZM metabolite, is a positively strong intestinal agonist that may result in the intestinal mobility experienced by patients after AZM administration. We confirmed that Deg-AZM can function strongly on intestinal peristalsis and identified transgelin as its potential molecular target. Furthermore, our pharmacological studies showed that the binding of Deg-AZM to transgelin enhanced the contractility of intestinal smooth muscle cells by facilitating the assembly of actin filaments into tight bundles and stress fibers. Specifically, Deg-AZM promoted intestinal peristaltic activity in wild-type mice but not in transgelin (−/−) mice. Moreover, Deg-AZM did not exert antibacterial activity and did not disrupt intestinal flora. Thus, Deg-AZM may become a potential drug for slow-transit constipation treatment. Elsevier 2020-08-14 /pmc/articles/PMC7479357/ /pubmed/32889431 http://dx.doi.org/10.1016/j.isci.2020.101464 Text en © 2020. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Zhong, Weilong Sun, Bo Ruan, Hao Yang, Guang Qian, Baoxin Cao, Hailong He, Lingfei Fan, Yunjing Roberts, Arthur G. Liu, Xiang Hu, Xuejiao Liang, Yuan Ye, Qing Yin, Tingting Wang, Bangmao Yang, Cheng Sun, Tao Zhou, Honggang Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function |
title | Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function |
title_full | Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function |
title_fullStr | Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function |
title_full_unstemmed | Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function |
title_short | Deglycosylated Azithromycin Targets Transgelin to Enhance Intestinal Smooth Muscle Function |
title_sort | deglycosylated azithromycin targets transgelin to enhance intestinal smooth muscle function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479357/ https://www.ncbi.nlm.nih.gov/pubmed/32889431 http://dx.doi.org/10.1016/j.isci.2020.101464 |
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