Cargando…
An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission
The endosomal sorting complex required for transport-III (ESCRT-III) catalyzes membrane fission from within membrane necks, a process that is essential for many cellular functions, from cell division to lysosome degradation and autophagy. How it breaks membranes, though, remains unknown. Here, we ch...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479521/ https://www.ncbi.nlm.nih.gov/pubmed/32814015 http://dx.doi.org/10.1016/j.cell.2020.07.021 |
_version_ | 1783580291168731136 |
---|---|
author | Pfitzner, Anna-Katharina Mercier, Vincent Jiang, Xiuyun Moser von Filseck, Joachim Baum, Buzz Šarić, Anđela Roux, Aurélien |
author_facet | Pfitzner, Anna-Katharina Mercier, Vincent Jiang, Xiuyun Moser von Filseck, Joachim Baum, Buzz Šarić, Anđela Roux, Aurélien |
author_sort | Pfitzner, Anna-Katharina |
collection | PubMed |
description | The endosomal sorting complex required for transport-III (ESCRT-III) catalyzes membrane fission from within membrane necks, a process that is essential for many cellular functions, from cell division to lysosome degradation and autophagy. How it breaks membranes, though, remains unknown. Here, we characterize a sequential polymerization of ESCRT-III subunits that, driven by a recruitment cascade and by continuous subunit-turnover powered by the ATPase Vps4, induces membrane deformation and fission. During this process, the exchange of Vps24 for Did2 induces a tilt in the polymer-membrane interface, which triggers transition from flat spiral polymers to helical filament to drive the formation of membrane protrusions, and ends with the formation of a highly constricted Did2-Ist1 co-polymer that we show is competent to promote fission when bound on the inside of membrane necks. Overall, our results suggest a mechanism of stepwise changes in ESCRT-III filament structure and mechanical properties via exchange of the filament subunits to catalyze ESCRT-III activity. |
format | Online Article Text |
id | pubmed-7479521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-74795212020-09-16 An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission Pfitzner, Anna-Katharina Mercier, Vincent Jiang, Xiuyun Moser von Filseck, Joachim Baum, Buzz Šarić, Anđela Roux, Aurélien Cell Article The endosomal sorting complex required for transport-III (ESCRT-III) catalyzes membrane fission from within membrane necks, a process that is essential for many cellular functions, from cell division to lysosome degradation and autophagy. How it breaks membranes, though, remains unknown. Here, we characterize a sequential polymerization of ESCRT-III subunits that, driven by a recruitment cascade and by continuous subunit-turnover powered by the ATPase Vps4, induces membrane deformation and fission. During this process, the exchange of Vps24 for Did2 induces a tilt in the polymer-membrane interface, which triggers transition from flat spiral polymers to helical filament to drive the formation of membrane protrusions, and ends with the formation of a highly constricted Did2-Ist1 co-polymer that we show is competent to promote fission when bound on the inside of membrane necks. Overall, our results suggest a mechanism of stepwise changes in ESCRT-III filament structure and mechanical properties via exchange of the filament subunits to catalyze ESCRT-III activity. Cell Press 2020-09-03 /pmc/articles/PMC7479521/ /pubmed/32814015 http://dx.doi.org/10.1016/j.cell.2020.07.021 Text en Crown Copyright © 2020 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Pfitzner, Anna-Katharina Mercier, Vincent Jiang, Xiuyun Moser von Filseck, Joachim Baum, Buzz Šarić, Anđela Roux, Aurélien An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission |
title | An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission |
title_full | An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission |
title_fullStr | An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission |
title_full_unstemmed | An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission |
title_short | An ESCRT-III Polymerization Sequence Drives Membrane Deformation and Fission |
title_sort | escrt-iii polymerization sequence drives membrane deformation and fission |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479521/ https://www.ncbi.nlm.nih.gov/pubmed/32814015 http://dx.doi.org/10.1016/j.cell.2020.07.021 |
work_keys_str_mv | AT pfitznerannakatharina anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT merciervincent anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT jiangxiuyun anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT moservonfilseckjoachim anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT baumbuzz anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT saricanđela anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT rouxaurelien anescrtiiipolymerizationsequencedrivesmembranedeformationandfission AT pfitznerannakatharina escrtiiipolymerizationsequencedrivesmembranedeformationandfission AT merciervincent escrtiiipolymerizationsequencedrivesmembranedeformationandfission AT jiangxiuyun escrtiiipolymerizationsequencedrivesmembranedeformationandfission AT moservonfilseckjoachim escrtiiipolymerizationsequencedrivesmembranedeformationandfission AT baumbuzz escrtiiipolymerizationsequencedrivesmembranedeformationandfission AT saricanđela escrtiiipolymerizationsequencedrivesmembranedeformationandfission AT rouxaurelien escrtiiipolymerizationsequencedrivesmembranedeformationandfission |