A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

To facilitate preclinical testing of T-cell receptors (TCRs) derived from tumor-reactive T-cell clones it is necessary to develop convenient and rapid cloning strategies for the generation of TCR expression constructs. Herein, we describe a pDONR™221 vector backbone allowing to generate Gateway™ com...

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Detalles Bibliográficos
Autores principales: Kropp, Korbinian N., Schäufele, Tim J., Fatho, Martina, Volkmar, Michael, Conradi, Roland, Theobald, Matthias, Wölfel, Thomas, Wölfel, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480877/
https://www.ncbi.nlm.nih.gov/pubmed/32903281
http://dx.doi.org/10.1371/journal.pone.0238875

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480877/
https://www.ncbi.nlm.nih.gov/pubmed/32903281
http://dx.doi.org/10.1371/journal.pone.0238875

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