Cargando…
Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study
BACKGROUND: Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (iPSCs) hold great promise in toxicological applications as well as in regenerative medicine. Previous efforts on hepatocyte differentiation have mostly relied on the use of growth factors (GFs) to recapitulat...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488531/ https://www.ncbi.nlm.nih.gov/pubmed/32917265 http://dx.doi.org/10.1186/s13287-020-01914-1 |
_version_ | 1783581711281422336 |
---|---|
author | Gao, Xiugong Li, Rong Cahan, Patrick Zhao, Yang Yourick, Jeffrey J. Sprando, Robert L. |
author_facet | Gao, Xiugong Li, Rong Cahan, Patrick Zhao, Yang Yourick, Jeffrey J. Sprando, Robert L. |
author_sort | Gao, Xiugong |
collection | PubMed |
description | BACKGROUND: Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (iPSCs) hold great promise in toxicological applications as well as in regenerative medicine. Previous efforts on hepatocyte differentiation have mostly relied on the use of growth factors (GFs) to recapitulate developmental signals under in vitro conditions. Recently, the use of small molecules (SMs) has emerged as an attractive tool to induce cell fate transition due to its superiority in terms of both quality and cost. However, HLCs derived using SMs have not been well characterized, especially on the transcriptome level. METHODS: HLCs were differentiated from human iPSCs using a protocol that only involves SMs and characterized by transcriptomic analysis using whole genome microarrays. RESULTS: HLCs derived using the SM protocol (HLC_SM) displayed specific hepatic marker expression and demonstrated key hepatic functions. Transcriptomic analysis of the SM-driven differentiation defined a hepatocyte differentiation track and characterized the expression of some key marker genes in major stages of hepatocyte differentiation. In addition, HLC_SM were scored with CellNet, a bioinformatics tool quantifying how closely engineered cell populations resemble their target cell type, and compared to primary human hepatocytes (PHHs), adult liver tissue, fetal liver tissue, HLCs differentiated using GFs (HLC_GF), and commercially available HLCs. Similar to HLC_GF, HLC_SM displayed a mixed phenotype of fetal and adult hepatocytes and had relatively low expression of metabolic enzymes, transporters, and nuclear receptors compared to PHHs. Finally, the differentially expressed genes in HLC_SM compared to HLC_GF and to PHHs were analyzed to identify pathways and upstream transcription regulators which could potentially be manipulated to improve the differentiation of HLCs. CONCLUSIONS: Overall, the present study demonstrated the usefulness of the SM-based hepatocyte differentiation method, offered new insights into the molecular basis of hepatogenesis and associated gene regulation, and suggested ways for further improvements in hepatocyte differentiation in order to obtain more mature HLCs that could be used in toxicological studies. |
format | Online Article Text |
id | pubmed-7488531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-74885312020-09-16 Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study Gao, Xiugong Li, Rong Cahan, Patrick Zhao, Yang Yourick, Jeffrey J. Sprando, Robert L. Stem Cell Res Ther Research BACKGROUND: Hepatocyte-like cells (HLCs) derived from human induced pluripotent stem cells (iPSCs) hold great promise in toxicological applications as well as in regenerative medicine. Previous efforts on hepatocyte differentiation have mostly relied on the use of growth factors (GFs) to recapitulate developmental signals under in vitro conditions. Recently, the use of small molecules (SMs) has emerged as an attractive tool to induce cell fate transition due to its superiority in terms of both quality and cost. However, HLCs derived using SMs have not been well characterized, especially on the transcriptome level. METHODS: HLCs were differentiated from human iPSCs using a protocol that only involves SMs and characterized by transcriptomic analysis using whole genome microarrays. RESULTS: HLCs derived using the SM protocol (HLC_SM) displayed specific hepatic marker expression and demonstrated key hepatic functions. Transcriptomic analysis of the SM-driven differentiation defined a hepatocyte differentiation track and characterized the expression of some key marker genes in major stages of hepatocyte differentiation. In addition, HLC_SM were scored with CellNet, a bioinformatics tool quantifying how closely engineered cell populations resemble their target cell type, and compared to primary human hepatocytes (PHHs), adult liver tissue, fetal liver tissue, HLCs differentiated using GFs (HLC_GF), and commercially available HLCs. Similar to HLC_GF, HLC_SM displayed a mixed phenotype of fetal and adult hepatocytes and had relatively low expression of metabolic enzymes, transporters, and nuclear receptors compared to PHHs. Finally, the differentially expressed genes in HLC_SM compared to HLC_GF and to PHHs were analyzed to identify pathways and upstream transcription regulators which could potentially be manipulated to improve the differentiation of HLCs. CONCLUSIONS: Overall, the present study demonstrated the usefulness of the SM-based hepatocyte differentiation method, offered new insights into the molecular basis of hepatogenesis and associated gene regulation, and suggested ways for further improvements in hepatocyte differentiation in order to obtain more mature HLCs that could be used in toxicological studies. BioMed Central 2020-09-11 /pmc/articles/PMC7488531/ /pubmed/32917265 http://dx.doi.org/10.1186/s13287-020-01914-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Gao, Xiugong Li, Rong Cahan, Patrick Zhao, Yang Yourick, Jeffrey J. Sprando, Robert L. Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
title | Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
title_full | Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
title_fullStr | Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
title_full_unstemmed | Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
title_short | Hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
title_sort | hepatocyte-like cells derived from human induced pluripotent stem cells using small molecules: implications of a transcriptomic study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488531/ https://www.ncbi.nlm.nih.gov/pubmed/32917265 http://dx.doi.org/10.1186/s13287-020-01914-1 |
work_keys_str_mv | AT gaoxiugong hepatocytelikecellsderivedfromhumaninducedpluripotentstemcellsusingsmallmoleculesimplicationsofatranscriptomicstudy AT lirong hepatocytelikecellsderivedfromhumaninducedpluripotentstemcellsusingsmallmoleculesimplicationsofatranscriptomicstudy AT cahanpatrick hepatocytelikecellsderivedfromhumaninducedpluripotentstemcellsusingsmallmoleculesimplicationsofatranscriptomicstudy AT zhaoyang hepatocytelikecellsderivedfromhumaninducedpluripotentstemcellsusingsmallmoleculesimplicationsofatranscriptomicstudy AT yourickjeffreyj hepatocytelikecellsderivedfromhumaninducedpluripotentstemcellsusingsmallmoleculesimplicationsofatranscriptomicstudy AT sprandorobertl hepatocytelikecellsderivedfromhumaninducedpluripotentstemcellsusingsmallmoleculesimplicationsofatranscriptomicstudy |