Cargando…
Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways
Thymoquinone (TQ), a natural anticancer agent exerts cytotoxic effects on several tumors by targeting multiple pathways, including apoptosis. Difluoromethylornithine (DFMO), an irreversible inhibitor of the ornithine decarboxylase (ODC) enzyme, has shown promising inhibitory activities in many cance...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488875/ https://www.ncbi.nlm.nih.gov/pubmed/32912061 http://dx.doi.org/10.1177/1533033820947489 |
_version_ | 1783581782584590336 |
---|---|
author | Alhosin, Mahmoud Razvi, Syed Shoeb I. Sheikh, Ryan A. Khan, Jalaluddin A. Zamzami, Mazin A. Choudhry, Hani |
author_facet | Alhosin, Mahmoud Razvi, Syed Shoeb I. Sheikh, Ryan A. Khan, Jalaluddin A. Zamzami, Mazin A. Choudhry, Hani |
author_sort | Alhosin, Mahmoud |
collection | PubMed |
description | Thymoquinone (TQ), a natural anticancer agent exerts cytotoxic effects on several tumors by targeting multiple pathways, including apoptosis. Difluoromethylornithine (DFMO), an irreversible inhibitor of the ornithine decarboxylase (ODC) enzyme, has shown promising inhibitory activities in many cancers including leukemia by decreasing the biosynthesis of the intracellular polyamines. The present study aimed to investigate the combinatorial cytotoxic effects of TQ and DFMO on human acute T lymphoblastic leukemia Jurkat cells and to determine the underlying mechanisms. Here, we show that the combination of DFMO and TQ significantly reduced cell viability and resulted in significant synergistic effects on apoptosis when compared to either DFMO or TQ alone. RNA-sequencing showed that many key epigenetic players including Ubiquitin-like containing PHD and Ring finger 1 (UHRF1) and its 2 partners DNA methyltransferase 1 (DNMT1) and histone deacetylase 1 (HDAC1) were down-regulated in DFMO-treated Jurkat cells. The combination of DFMO and TQ dramatically decreased the expression of UHRF1, DNMT1 and HDAC1 genes compared to either DFMO or TQ alone. UHRF1 knockdown led to a decrease in Jurkat cell viability. In conclusion, these results suggest that the combination of DFMO and TQ could be a promising new strategy for the treatment of human acute T lymphoblastic leukemia by targeting the epigenetic code. |
format | Online Article Text |
id | pubmed-7488875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-74888752020-09-21 Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways Alhosin, Mahmoud Razvi, Syed Shoeb I. Sheikh, Ryan A. Khan, Jalaluddin A. Zamzami, Mazin A. Choudhry, Hani Technol Cancer Res Treat Original Article Thymoquinone (TQ), a natural anticancer agent exerts cytotoxic effects on several tumors by targeting multiple pathways, including apoptosis. Difluoromethylornithine (DFMO), an irreversible inhibitor of the ornithine decarboxylase (ODC) enzyme, has shown promising inhibitory activities in many cancers including leukemia by decreasing the biosynthesis of the intracellular polyamines. The present study aimed to investigate the combinatorial cytotoxic effects of TQ and DFMO on human acute T lymphoblastic leukemia Jurkat cells and to determine the underlying mechanisms. Here, we show that the combination of DFMO and TQ significantly reduced cell viability and resulted in significant synergistic effects on apoptosis when compared to either DFMO or TQ alone. RNA-sequencing showed that many key epigenetic players including Ubiquitin-like containing PHD and Ring finger 1 (UHRF1) and its 2 partners DNA methyltransferase 1 (DNMT1) and histone deacetylase 1 (HDAC1) were down-regulated in DFMO-treated Jurkat cells. The combination of DFMO and TQ dramatically decreased the expression of UHRF1, DNMT1 and HDAC1 genes compared to either DFMO or TQ alone. UHRF1 knockdown led to a decrease in Jurkat cell viability. In conclusion, these results suggest that the combination of DFMO and TQ could be a promising new strategy for the treatment of human acute T lymphoblastic leukemia by targeting the epigenetic code. SAGE Publications 2020-09-10 /pmc/articles/PMC7488875/ /pubmed/32912061 http://dx.doi.org/10.1177/1533033820947489 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Alhosin, Mahmoud Razvi, Syed Shoeb I. Sheikh, Ryan A. Khan, Jalaluddin A. Zamzami, Mazin A. Choudhry, Hani Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways |
title | Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways |
title_full | Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways |
title_fullStr | Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways |
title_full_unstemmed | Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways |
title_short | Thymoquinone and Difluoromethylornithine (DFMO) Synergistically Induce Apoptosis of Human Acute T Lymphoblastic Leukemia Jurkat Cells Through the Modulation of Epigenetic Pathways |
title_sort | thymoquinone and difluoromethylornithine (dfmo) synergistically induce apoptosis of human acute t lymphoblastic leukemia jurkat cells through the modulation of epigenetic pathways |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488875/ https://www.ncbi.nlm.nih.gov/pubmed/32912061 http://dx.doi.org/10.1177/1533033820947489 |
work_keys_str_mv | AT alhosinmahmoud thymoquinoneanddifluoromethylornithinedfmosynergisticallyinduceapoptosisofhumanacutetlymphoblasticleukemiajurkatcellsthroughthemodulationofepigeneticpathways AT razvisyedshoebi thymoquinoneanddifluoromethylornithinedfmosynergisticallyinduceapoptosisofhumanacutetlymphoblasticleukemiajurkatcellsthroughthemodulationofepigeneticpathways AT sheikhryana thymoquinoneanddifluoromethylornithinedfmosynergisticallyinduceapoptosisofhumanacutetlymphoblasticleukemiajurkatcellsthroughthemodulationofepigeneticpathways AT khanjalaluddina thymoquinoneanddifluoromethylornithinedfmosynergisticallyinduceapoptosisofhumanacutetlymphoblasticleukemiajurkatcellsthroughthemodulationofepigeneticpathways AT zamzamimazina thymoquinoneanddifluoromethylornithinedfmosynergisticallyinduceapoptosisofhumanacutetlymphoblasticleukemiajurkatcellsthroughthemodulationofepigeneticpathways AT choudhryhani thymoquinoneanddifluoromethylornithinedfmosynergisticallyinduceapoptosisofhumanacutetlymphoblasticleukemiajurkatcellsthroughthemodulationofepigeneticpathways |