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Methylase-assisted subcloning for high throughput BioBrick assembly

The BioBrick standard makes possible iterated pairwise assembly of cloned parts without any depletion of unique restriction sites. Every part that conforms to the standard is compatible with every other part, thereby fostering a worldwide user community. The assembly methods, however, are labor inte...

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Detalles Bibliográficos
Autor principal: Matsumura, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7489255/
https://www.ncbi.nlm.nih.gov/pubmed/32974095
http://dx.doi.org/10.7717/peerj.9841