MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3
PURPOSE: This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 (PANX3). METHODS: qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexp...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490058/ https://www.ncbi.nlm.nih.gov/pubmed/32982413 http://dx.doi.org/10.2147/CMAR.S260149 |
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author | Sun, Shengliang Fu, Lei Wang, Gen Wang, Jianli Xu, Liping |
author_facet | Sun, Shengliang Fu, Lei Wang, Gen Wang, Jianli Xu, Liping |
author_sort | Sun, Shengliang |
collection | PubMed |
description | PURPOSE: This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 (PANX3). METHODS: qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed to detect cell migration and invasion. The protein expression of cleave-caspase-3 and MMP-2/-9 was detected by Western blot. The target relationship between miR-431-5p and PANX3 was predicated by ENCORI and identified by DLR assay. The anti-tumor effect of miR-431-5p was further analyzed in a xenograft tumor model in mice. RESULTS: MiR-431-5p expression was down-regulated in OS tissues and negatively correlated with lymph node metastasis and TNM stage. Over-expression of miR-431-5p induced cell apoptosis, inhibited cell proliferation, migration and invasion, up-regulated cleave-caspase-3, and down-regulated MMP-2 and -9 in OS cells. Over-expression of miR-431-5p also inhibited the growth of tumor xenografts in mice. In addition, PANX3 was a target of miR-431-5p. Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on U2OS and HOS cells. CONCLUSION: Up-regulation of miR-431-5p suppressed the tumorigenesis of OS via targeting PANX3. |
format | Online Article Text |
id | pubmed-7490058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-74900582020-09-24 MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 Sun, Shengliang Fu, Lei Wang, Gen Wang, Jianli Xu, Liping Cancer Manag Res Original Research PURPOSE: This study aimed to evaluate the regulatory role of miR-431-5p on the tumorigenesis of osteosarcoma (OS) and the underlying mechanism involving pannexin 3 (PANX3). METHODS: qRT-PCR was applied to measure the expression of miR-431-5p in OS tissues and cells. PANX3 and miR-431-5p were overexpressed in U2OS and HOS cells. The cell viability and apoptosis were determined by MTT and FITC/PI double staining assay, respectively. Transwell assay was performed to detect cell migration and invasion. The protein expression of cleave-caspase-3 and MMP-2/-9 was detected by Western blot. The target relationship between miR-431-5p and PANX3 was predicated by ENCORI and identified by DLR assay. The anti-tumor effect of miR-431-5p was further analyzed in a xenograft tumor model in mice. RESULTS: MiR-431-5p expression was down-regulated in OS tissues and negatively correlated with lymph node metastasis and TNM stage. Over-expression of miR-431-5p induced cell apoptosis, inhibited cell proliferation, migration and invasion, up-regulated cleave-caspase-3, and down-regulated MMP-2 and -9 in OS cells. Over-expression of miR-431-5p also inhibited the growth of tumor xenografts in mice. In addition, PANX3 was a target of miR-431-5p. Over-expression of PANX3 reversed the anti-tumor effect of miR-431-5p mimics on U2OS and HOS cells. CONCLUSION: Up-regulation of miR-431-5p suppressed the tumorigenesis of OS via targeting PANX3. Dove 2020-09-08 /pmc/articles/PMC7490058/ /pubmed/32982413 http://dx.doi.org/10.2147/CMAR.S260149 Text en © 2020 Sun et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Sun, Shengliang Fu, Lei Wang, Gen Wang, Jianli Xu, Liping MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
title | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
title_full | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
title_fullStr | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
title_full_unstemmed | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
title_short | MicroRNA-431-5p Inhibits the Tumorigenesis of Osteosarcoma Through Targeting PANX3 |
title_sort | microrna-431-5p inhibits the tumorigenesis of osteosarcoma through targeting panx3 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490058/ https://www.ncbi.nlm.nih.gov/pubmed/32982413 http://dx.doi.org/10.2147/CMAR.S260149 |
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