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Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture
Systemic and local posttraumatic responses are often monitored on mRNA expression level using quantitative real-time PCR (qRT-PCR), which requires normalisation to adjust for confounding sources of variability. Normalisation requests reference (housekeeping) genes stable throughout time and divergen...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490670/ https://www.ncbi.nlm.nih.gov/pubmed/32929099 http://dx.doi.org/10.1038/s41598-020-71895-x |