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Nuclease-mediated depletion biases in ribosome footprint profiling libraries

Ribosome footprint profiling is a high-throughput sequencing–based technique that provides detailed and global views of translation in living cells. An essential part of this technology is removal of unwanted, normally very abundant, ribosomal RNA sequences that dominate libraries and increase seque...

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Detalles Bibliográficos
Autores principales: Zinshteyn, Boris, Wangen, Jamie R., Hua, Boyang, Green, Rachel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7491325/
https://www.ncbi.nlm.nih.gov/pubmed/32503920
http://dx.doi.org/10.1261/rna.075523.120