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Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of molecular chaperon HAC1 was constructed. The amoun...

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Detalles Bibliográficos
Autores principales: Song, Wen, Zhang, Nuo, Yang, Mo, Zhou, Yuling, He, Nisha, Zhang, Guimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493387/
https://www.ncbi.nlm.nih.gov/pubmed/32933546
http://dx.doi.org/10.1186/s12934-020-01440-y