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An internally eGFP‐tagged α‐adaptin is a fully functional and improved fiduciary marker for clathrin‐coated pit dynamics

Clathrin mediated endocytosis (CME) has been extensively studied in living cells by quantitative total internal reflection fluorescence microscopy (TIRFM). Fluorescent protein fusions to subunits of the major coat proteins, clathrin light chains or the heterotetrameric adaptor protein (AP2) complexe...

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Detalles Bibliográficos
Autores principales: Mino, Rosa E., Chen, Zhiming, Mettlen, Marcel, Schmid, Sandra L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons A/S 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495412/
https://www.ncbi.nlm.nih.gov/pubmed/32657003
http://dx.doi.org/10.1111/tra.12755