The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability

Nucleosome positioning and stability affect gene regulation in eukaryotic chromatin. Histone H2A.Z is an evolutionally conserved histone variant that forms mobile and unstable nucleosomes in vivo and in vitro. In the present study, we reconstituted nucleosomes containing human H2A.Z.1 mutants, in wh...

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Autores principales: Sato, Shoko, Tanaka, Naoki, Arimura, Yasuhiro, Kujirai, Tomoya, Kurumizaka, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496805/
https://www.ncbi.nlm.nih.gov/pubmed/32500630
http://dx.doi.org/10.1111/gtc.12791
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author Sato, Shoko
Tanaka, Naoki
Arimura, Yasuhiro
Kujirai, Tomoya
Kurumizaka, Hitoshi
author_facet Sato, Shoko
Tanaka, Naoki
Arimura, Yasuhiro
Kujirai, Tomoya
Kurumizaka, Hitoshi
author_sort Sato, Shoko
collection PubMed
description Nucleosome positioning and stability affect gene regulation in eukaryotic chromatin. Histone H2A.Z is an evolutionally conserved histone variant that forms mobile and unstable nucleosomes in vivo and in vitro. In the present study, we reconstituted nucleosomes containing human H2A.Z.1 mutants, in which the N‐terminal or C‐terminal half of H2A.Z.1 was replaced by the corresponding canonical H2A region. We found that the N‐terminal portion of H2A.Z.1 is involved in flexible nucleosome positioning, whereas the C‐terminal portion leads to weak H2A.Z.1‐H2B association in the nucleosome. These results indicate that the N‐terminal and C‐terminal portions are independently responsible for the H2A.Z.1 nucleosome characteristics.
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spelling pubmed-74968052020-09-25 The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability Sato, Shoko Tanaka, Naoki Arimura, Yasuhiro Kujirai, Tomoya Kurumizaka, Hitoshi Genes Cells Original Articles Nucleosome positioning and stability affect gene regulation in eukaryotic chromatin. Histone H2A.Z is an evolutionally conserved histone variant that forms mobile and unstable nucleosomes in vivo and in vitro. In the present study, we reconstituted nucleosomes containing human H2A.Z.1 mutants, in which the N‐terminal or C‐terminal half of H2A.Z.1 was replaced by the corresponding canonical H2A region. We found that the N‐terminal portion of H2A.Z.1 is involved in flexible nucleosome positioning, whereas the C‐terminal portion leads to weak H2A.Z.1‐H2B association in the nucleosome. These results indicate that the N‐terminal and C‐terminal portions are independently responsible for the H2A.Z.1 nucleosome characteristics. John Wiley and Sons Inc. 2020-07-22 2020-08 /pmc/articles/PMC7496805/ /pubmed/32500630 http://dx.doi.org/10.1111/gtc.12791 Text en © 2020 The Authors. Genes to Cells published by Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Sato, Shoko
Tanaka, Naoki
Arimura, Yasuhiro
Kujirai, Tomoya
Kurumizaka, Hitoshi
The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability
title The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability
title_full The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability
title_fullStr The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability
title_full_unstemmed The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability
title_short The N‐terminal and C‐terminal halves of histone H2A.Z independently function in nucleosome positioning and stability
title_sort n‐terminal and c‐terminal halves of histone h2a.z independently function in nucleosome positioning and stability
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496805/
https://www.ncbi.nlm.nih.gov/pubmed/32500630
http://dx.doi.org/10.1111/gtc.12791
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