Enhancing the promiscuity of a member of the Caspase protease family by rational design

The N‐terminal cleavage of fusion tags to restore the native N‐terminus of recombinant proteins is a challenging task and up to today, protocols need to be optimized for different proteins individually. Within this work, we present a novel protease that was designed in‐silico to yield enhanced promi...

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Detalles Bibliográficos
Autores principales: Öhlknecht, Christoph, Petrov, Drazen, Engele, Petra, Kröß, Christina, Sprenger, Bernhard, Fischer, Andreas, Lingg, Nico, Schneider, Rainer, Oostenbrink, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2020
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497161/
https://www.ncbi.nlm.nih.gov/pubmed/32432825
http://dx.doi.org/10.1002/prot.25950

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497161/
https://www.ncbi.nlm.nih.gov/pubmed/32432825
http://dx.doi.org/10.1002/prot.25950

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