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Analysis of Brightness of a Single Fluorophore for Quantitative Characterization of Biochemical Reactions
[Image: see text] Intrinsic molecular brightness (MB) is a number of emitted photons per second per molecule. When a substrate labeled by a fluorophore and a second unlabeled substrate form a complex in solution, the MB of the fluorophore changes. Here we use this change to determine the equilibrium...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497653/ https://www.ncbi.nlm.nih.gov/pubmed/32059107 http://dx.doi.org/10.1021/acs.jpcb.0c00770 |