Cargando…
Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds
In recent times, there has been a growing interest in the exploration of antioxidants and global trend toward the usage of seaweeds in the food industries. The low molecular weight up to 14 kDa sulfated polysaccharides of seaweeds (Portieria hornemannii, Spyridia hypnoides, Asparagopsis taxiformis,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498196/ http://dx.doi.org/10.1007/s11694-020-00661-4 |
_version_ | 1783583458352693248 |
---|---|
author | Arunkumar, K. Raja, Rathinam Kumar, V. B. Sameer Joseph, Ashna Shilpa, T. Carvalho, Isabel S. |
author_facet | Arunkumar, K. Raja, Rathinam Kumar, V. B. Sameer Joseph, Ashna Shilpa, T. Carvalho, Isabel S. |
author_sort | Arunkumar, K. |
collection | PubMed |
description | In recent times, there has been a growing interest in the exploration of antioxidants and global trend toward the usage of seaweeds in the food industries. The low molecular weight up to 14 kDa sulfated polysaccharides of seaweeds (Portieria hornemannii, Spyridia hypnoides, Asparagopsis taxiformis, Centroceras clavulatum and Padina pavonica) were evaluated for in vitro antioxidant activities and cytotoxic assay using HeLa cell line and also characterized by FTIR. The high yield (7.74% alga dry wt.) of sulfated polysaccharide was observed in P. hornemannii followed by S. hypnoides (0.69%), C. clavulaum (0.55%) and A. taxiformis (0.17%). In the brown seaweed P. pavonica, the sulfated polysaccharide yield was 2.07%. High amount of sulfate was recorded in the polysaccharide of A. taxiformis followed by C. clavulaum, P. pavonica, S. hypnoides and P. hornemannii as indicative for bioactivity. The FTIR spectroscopic analysis supports the sulfated polysaccharides of S. hypnoides, C. clavulatum and A. taxiformis are similar to agar polymer whereas the spectral characteristics of P. hornemannii have similarities to carrageenan. The higher DPPH activity and reducing power were recorded in the polysaccharide of brown seaweed P. pavonica than the red seaweeds as follows: DPPH activities: S. hypnoides > A. taxiformis > C. clavulatum > P. hornimanii; Reducing power: A. taxiformis > P. hornimanii > S. hypnoides > C. clavulatum. The polysaccharide fractions contain up to 14 kDa from red seaweeds P. hornemannii and S. hypnoides followed by brown seaweed P. pavonica exhibit cytotoxic activity in HeLa cancer cell line (and are similar to structural properties of carrageenan extracted from P. hornemannii). The low molecular weight agar like polymer of S. hypnoides and alginate like brown seaweed P. pavonica showing better in vitro antioxidant activities that are capable of exhibiting cytotoxicity against HeLa cell line can be taken up further in-depth investigation for nutraceutical study. |
format | Online Article Text |
id | pubmed-7498196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-74981962020-09-18 Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds Arunkumar, K. Raja, Rathinam Kumar, V. B. Sameer Joseph, Ashna Shilpa, T. Carvalho, Isabel S. Food Measure Original Paper In recent times, there has been a growing interest in the exploration of antioxidants and global trend toward the usage of seaweeds in the food industries. The low molecular weight up to 14 kDa sulfated polysaccharides of seaweeds (Portieria hornemannii, Spyridia hypnoides, Asparagopsis taxiformis, Centroceras clavulatum and Padina pavonica) were evaluated for in vitro antioxidant activities and cytotoxic assay using HeLa cell line and also characterized by FTIR. The high yield (7.74% alga dry wt.) of sulfated polysaccharide was observed in P. hornemannii followed by S. hypnoides (0.69%), C. clavulaum (0.55%) and A. taxiformis (0.17%). In the brown seaweed P. pavonica, the sulfated polysaccharide yield was 2.07%. High amount of sulfate was recorded in the polysaccharide of A. taxiformis followed by C. clavulaum, P. pavonica, S. hypnoides and P. hornemannii as indicative for bioactivity. The FTIR spectroscopic analysis supports the sulfated polysaccharides of S. hypnoides, C. clavulatum and A. taxiformis are similar to agar polymer whereas the spectral characteristics of P. hornemannii have similarities to carrageenan. The higher DPPH activity and reducing power were recorded in the polysaccharide of brown seaweed P. pavonica than the red seaweeds as follows: DPPH activities: S. hypnoides > A. taxiformis > C. clavulatum > P. hornimanii; Reducing power: A. taxiformis > P. hornimanii > S. hypnoides > C. clavulatum. The polysaccharide fractions contain up to 14 kDa from red seaweeds P. hornemannii and S. hypnoides followed by brown seaweed P. pavonica exhibit cytotoxic activity in HeLa cancer cell line (and are similar to structural properties of carrageenan extracted from P. hornemannii). The low molecular weight agar like polymer of S. hypnoides and alginate like brown seaweed P. pavonica showing better in vitro antioxidant activities that are capable of exhibiting cytotoxicity against HeLa cell line can be taken up further in-depth investigation for nutraceutical study. Springer US 2020-09-17 2021 /pmc/articles/PMC7498196/ http://dx.doi.org/10.1007/s11694-020-00661-4 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Arunkumar, K. Raja, Rathinam Kumar, V. B. Sameer Joseph, Ashna Shilpa, T. Carvalho, Isabel S. Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
title | Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
title_full | Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
title_fullStr | Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
title_full_unstemmed | Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
title_short | Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
title_sort | antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498196/ http://dx.doi.org/10.1007/s11694-020-00661-4 |
work_keys_str_mv | AT arunkumark antioxidantandcytotoxicactivitiesofsulfatedpolysaccharidesfromfivedifferentedibleseaweeds AT rajarathinam antioxidantandcytotoxicactivitiesofsulfatedpolysaccharidesfromfivedifferentedibleseaweeds AT kumarvbsameer antioxidantandcytotoxicactivitiesofsulfatedpolysaccharidesfromfivedifferentedibleseaweeds AT josephashna antioxidantandcytotoxicactivitiesofsulfatedpolysaccharidesfromfivedifferentedibleseaweeds AT shilpat antioxidantandcytotoxicactivitiesofsulfatedpolysaccharidesfromfivedifferentedibleseaweeds AT carvalhoisabels antioxidantandcytotoxicactivitiesofsulfatedpolysaccharidesfromfivedifferentedibleseaweeds |