DropSynth 2.0: high-fidelity multiplexed gene synthesis in emulsions

Multiplexed assays allow functional testing of large synthetic libraries of genetic elements, but are limited by the designability, length, fidelity and scale of the input DNA. Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compartmentalizing and assembling...

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Detalles Bibliográficos
Autores principales: Sidore, Angus M, Plesa, Calin, Samson, Joyce A, Lubock, Nathan B, Kosuri, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498354/
https://www.ncbi.nlm.nih.gov/pubmed/32692349
http://dx.doi.org/10.1093/nar/gkaa600

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7498354/
https://www.ncbi.nlm.nih.gov/pubmed/32692349
http://dx.doi.org/10.1093/nar/gkaa600

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