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Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription

The control of viral outbreaks requires nucleic acid diagnostic tests that are sensitive, simple and fast. Here, we report a highly sensitive and specific one-pot assay for the fluorescence-based detection of RNA from pathogens. The assay, which can be performed within 30–50 min of incubation time a...

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Detalles Bibliográficos
Autores principales: Woo, Chang Ha, Jang, Sungho, Shin, Giyoung, Jung, Gyoo Yeol, Lee, Jeong Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499000/
https://www.ncbi.nlm.nih.gov/pubmed/32948855
http://dx.doi.org/10.1038/s41551-020-00617-5