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Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral patho...

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Detalles Bibliográficos
Autores principales: Huggett, Jim F, Benes, Vladimir, Bustin, Stephen A, Garson, Jeremy A, Harris, Karthyn, Kammel, Martin, Kubista, Mikael, McHugh, Timothy D, Moran-Gilad, Jacob, Nolan, Tania, Pfaffl, Michael W, Salit, Marc, Shipley, Greg, Vallone, Peter M, Vandesompele, Jo, Wittwer, Carl, Zeichhardt, Heinz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499524/
https://www.ncbi.nlm.nih.gov/pubmed/32894756
http://dx.doi.org/10.1093/clinchem/hvaa214