Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral patho...

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Detalles Bibliográficos
Autores principales: Huggett, Jim F, Benes, Vladimir, Bustin, Stephen A, Garson, Jeremy A, Harris, Karthyn, Kammel, Martin, Kubista, Mikael, McHugh, Timothy D, Moran-Gilad, Jacob, Nolan, Tania, Pfaffl, Michael W, Salit, Marc, Shipley, Greg, Vallone, Peter M, Vandesompele, Jo, Wittwer, Carl, Zeichhardt, Heinz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Opinion
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7499524/
https://www.ncbi.nlm.nih.gov/pubmed/32894756
http://dx.doi.org/10.1093/clinchem/hvaa214
Descripción
Sumario:Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross react with similar but distinct viral pathogens, such as the coronaviruses associated with the common cold, and should perform with very high analytical sensitivity. This analytical performance is predicated on the ability of the method to detect the presence of the selected nucleic acid target, without detection of a false positive signal.

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